cDNA cloning of an adult male putative lipocalin specific to tergal gland aphrodisiac secretion in an insect (Leucophaea maderae)
| Autor: | Rémy Brossut, Jean Delachambre, Hervé Bouhin, Ahmed Korchi |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
DNA Complementary Sequence analysis Recombinant Fusion Proteins Molecular Sequence Data Biophysics Gene Expression Cuticle surface protein Cockroaches Lipocalin Biology Biochemistry law.invention Cockroach Structural Biology law Complementary DNA Escherichia coli Genetics Animals Humans Secretion Sex behavior Amino Acid Sequence RNA Messenger Northern blot Cloning Molecular Sex Attractants Molecular Biology Messenger RNA Base Sequence Sequence Homology Amino Acid integumentary system Edman degradation Cell Biology Tergal gland Molecular biology Recombinant DNA Insect Proteins |
| Zdroj: | FEBS Letters. 449:125-128 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(99)00414-7 |
| Popis: | Lma-P22 is a cuticular surface protein specific to the tergal gland secretion of Leucophaea maderae adult males which is ingested by females just before copulation. The complete Lma-P22 cDNA sequence was determined by RT-PCR using primers based on Edman degradation fragments. The recombinant protein expressed in Escherichia coli was recognized by an anti-Lma-P22 antibody. Northern blot analysis indicates that the corresponding mRNA is transcribed only in the epidermis of male tergites. Sequence analysis indicated that Lma-P22 deduced protein belongs to the lipocalin family. Lipocalins are extracellular proteins which carry hydrophobic compounds and some of them can bind sexual pheromone in vertebrates. Lma-P22 is the first example of a lipocalin-like protein involved in insect sexual behavior. |
| Databáze: | OpenAIRE |
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