Phenotyping of leukocytes and leukocyte-derived extracellular vesicles
| Autor: | Kim Varming, Anne Louise Revenfeld, Rikke Bæk, Lotte Hatting Pugholm, Malene Jørgensen, Evo Kristina Lindersson Søndergaard |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
0301 basic medicine Cell type Article Subject Immunology Cell Biology Immunophenotyping Extracellular Vesicles 03 medical and health sciences Immune system Antigen Antigens CD Leukocytes medicine Humans Immunology and Allergy Cell Lineage Cells Cultured CD63 General Medicine Phenotype Cell biology 030104 developmental biology medicine.anatomical_structure Case-Control Studies lcsh:RC581-607 Biomarkers Research Article CD81 |
| Zdroj: | Pugholm, L H, Bæk, R, Søndergaard, E K L, Revenfeld, A L, Jørgensen, M M & Varming, K 2016, ' Phenotyping of leukocytes and leukocyte-derived extracellular vesicles ', Journal of Extracellular Vesicles, vol. 5, no. Suppl. 1, PF3.08, pp. 149 . https://doi.org/10.3402/jev.v5.31552 Pugholm, L H, Bæk, R, Søndergaard, E K L, Revenfeld, A L, Jørgensen, M & Varming, K 2016, ' Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles ', Clinical & Developmental Immunology, vol. 2016, 6391264 . https://doi.org/10.1155/2016/6391264 Journal of Immunology Research, Vol 2016 (2016) Journal of Immunology Research Pugholm, L H, Bæk, R, Søndergaard, E K L, Revenfeld, A L S, Jørgensen, M M & Varming, K 2016, ' Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles ', Journal of Immunology Research, vol. 2016, 6391264 . https://doi.org/10.1155/2016/6391264 |
| DOI: | 10.3402/jev.v5.31552 |
| Popis: | Extracellular vesicles (EVs) have a demonstrated involvement in modulating the immune system. It has been proposed that EVs could be used as biomarkers for detection of inflammatory and immunological disorders. Consequently, it is of great interest to investigate EVs in more detail with focus on immunological markers. In this study, five major leukocyte subpopulations and the corresponding leukocyte-derived EVs were phenotyped with focus on selected immunological lineage-specific markers and selected vesicle-related markers. The leukocyte-derived EVs displayed phenotypic differences in the 34 markers investigated. The majority of the lineage-specific markers used for identification of the parent cell types could not be detected on EVs released from monocultures of the associated cell types. In contrast, the vesicular presentation of CD9, CD63, and CD81 correlated to the cell surface expression of these markers, however, with few exceptions. Furthermore, the cellular expression of CD9, CD63, and CD81 varied between leukocytes present in whole blood and cultured leukocytes. In summary, these data demonstrate that the cellular and vesicular presentation of selected lineage-specific and vesicle-related markers may differ, supporting the accumulating observations that sorting of molecular cargo into EVs is tightly controlled. |
| Databáze: | OpenAIRE |
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