Physiological responses of Saccharomyces cerevisiae to industrially relevant conditions
| Autor: | Mandy Hulst, Xavier D. V. Hakkaart, Anissa el Masoudi, Eveline Peuscher, Yaya Liu, Walter M. van Gulik, Jack T. Pronk, Pascale Daran-Lapujade |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cell Saccharomyces cerevisiae Carboxylic Acids Bioengineering Chemostat yeast 01 natural sciences Applied Microbiology and Biotechnology Article Systems Biotechnology ARTICLES Industrial Microbiology 03 medical and health sciences chemistry.chemical_compound zero‐growth 010608 biotechnology medicine Glycerol zero-growth biology acid stress carbon dioxide Hydrogen-Ion Concentration biology.organism_classification Metabolic Flux Analysis Yeast 030104 developmental biology medicine.anatomical_structure Metabolic Engineering chemistry Biochemistry Succinic acid Carbon dioxide carboxylic acid Signal transduction Transcriptome Biotechnology |
| Zdroj: | Biotechnology and Bioengineering, 117(3) Biotechnology and Bioengineering |
| ISSN: | 0006-3592 |
| DOI: | 10.1002/bit.27210 |
| Popis: | Engineered strains of Saccharomyces cerevisiae are used for industrial production of succinic acid. Optimal process conditions for dicarboxylic‐acid yield and recovery include slow growth, low pH, and high CO2. To quantify and understand how these process parameters affect yeast physiology, this study investigates individual and combined impacts of low pH (3.0) and high CO2 (50%) on slow‐growing chemostat and retentostat cultures of the reference strain S. cerevisiae CEN.PK113‐7D. Combined exposure to low pH and high CO2 led to increased maintenance‐energy requirements and death rates in aerobic, glucose‐limited cultures. Further experiments showed that these effects were predominantly caused by low pH. Growth under ammonium‐limited, energy‐excess conditions did not aggravate or ameliorate these adverse impacts. Despite the absence of a synergistic effect of low pH and high CO2 on physiology, high CO2 strongly affected genome‐wide transcriptional responses to low pH. Interference of high CO2 with low‐pH signaling is consistent with low‐pH and high‐CO2 signals being relayed via common (MAPK) signaling pathways, notably the cell wall integrity, high‐osmolarity glycerol, and calcineurin pathways. This study highlights the need to further increase robustness of cell factories to low pH for carboxylic‐acid production, even in organisms that are already applied at industrial scale. This study dissects adverse physiological effects of low pH, high CO2, and slow growth on S. cerevisiae and highlights the need to further increase robustness of cell factories to low pH for carboxylic‐acid production. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text