A HPLC-MS/MS method for screening of selected antibiotic adulterants in herbal drugs
| Autor: | Christopher Johnson Mwankuna, Grâce Ange Uwamaliya, Eliapenda Elisante Mariki, Faith Mabiki, Hamisi M. Malebo, Robinson Mdegela, Bjarne Styrishave |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Analytical Methods. 14:1060-1068 |
| ISSN: | 1759-9679 1759-9660 |
| Popis: | The use of herbal products adulterated with conventional drugs increases the risk of developing microbial resistance and causes herb-to-drug interaction, leading to severe clinical consequences. The complex nature of herbal products has been a challenge for the unambiguous identification of adulterants. The improved analytical selectivity and sensitivity of hyphenated techniques such as high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) enable the confirmatory screening of adulterants in herbal products. Simultaneous screening of adulterants is necessary and efficient because it has been established that more than one chemical adulterant may be present in one herbal product. An HPLC-MS/MS method for the simultaneous detection and quantification of amoxicillin, ampicillin, metronidazole, ciprofloxacin, sulfamethoxazole, and trimethoprim in powdered herbal drugs was developed. Deuterated metronidazole-d3, trimethoprim-d3, ciprofloxacin-d8, and sulfamethoxazole-d4 were used as internal standards (ISs). For each analyte, two transitions were monitored using protonated molecules as precursor ions. The extraction of analytes from herbal products was performed using a simple methanol : water : formic acid (90 : 10 : 0.05, v/v) extraction solvent. Chromatographic separation was done in a gradient of 0.01% formic acid in methanol and 0.01% formic acid in MilliQ water. The calibration curves were linear ( |
| Databáze: | OpenAIRE |
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