Directed deletion of a yeast transfer RNA intervening sequence
| Autor: | Monica Schold, John Abelson, Peter F. Johnson, Keiichi Itakura, R B Wallace, S Tanaka |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Genetics
Multidisciplinary Base Sequence Intron DNA Recombinant Nucleic Acid Precursors RNA Fungal Saccharomyces cerevisiae Biology Gene product Exon Suppression Genetic Genes RNA Transfer Regulatory sequence RNA splicing Gene cluster Mutation Tyrosine Chromosome Deletion Gene Regulator gene Plasmids |
| Zdroj: | Science (New York, N.Y.). 209(4463) |
| ISSN: | 0036-8075 |
| Popis: | Many eukaryotic genes contain intevening sequences, segments of DNA that interrupt the continuity of the gene. They are removed from RNA transcripts of the gene by a process known as splicing. The intervening sequence in a yeast tyrosine transfer RNA (tRNA Tyr) suppressor gene was deleted in order to test its role in the expression of the gene. The altered gene and its parent were introduced into yeast by transformation. Both genes exhibited suppressor function, showing that the intervening sequence is not absolutely essential for the expression of this gene. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|