Enhanced acetic acid stress tolerance and ethanol production in
| Autor: | Xin-Qing Zhao, Ya-jie Tang, Zongbao K. Zhao, Mingming Zhang, Fengwu Bai, Muhammad Aamer Mehmood, Liang Xiong |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
lcsh:Biotechnology Saccharomyces cerevisiae Management Monitoring Policy and Law 01 natural sciences Applied Microbiology and Biotechnology lcsh:Fuel Metabolic engineering 03 medical and health sciences chemistry.chemical_compound Acetic acid lcsh:TP315-360 Biosynthesis lcsh:TP248.13-248.65 010608 biotechnology Purine metabolism ADE17 Amino acid synthesis 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Renewable Energy Sustainability and the Environment Chemistry Research Yeast stress tolerance biology.organism_classification de novo purine biosynthesis Yeast Amino acid General Energy Biochemistry Global amino acid profiles Biotechnology |
| Zdroj: | Biotechnology for Biofuels Biotechnology for Biofuels, Vol 12, Iss 1, Pp 1-13 (2019) |
| ISSN: | 1754-6834 |
| Popis: | Background Yeast strains that are tolerant to multiple environmental stresses are highly desired for various industrial applications. Despite great efforts in identifying key genes involved in stress tolerance of budding yeast Saccharomyces cerevisiae, the effects of de novo purine biosynthesis genes on yeast stress tolerance are still not well explored. Our previous studies showed that zinc sulfate addition improved yeast acetic acid tolerance, and key genes involved in yeast stress tolerance were further investigated in this study. Results Three genes involved in de novo purine biosynthesis, namely, ADE1, ADE13, and ADE17, showed significantly increased transcription levels by zinc sulfate supplementation under acetic acid stress, and overexpression of these genes in S. cerevisiae BY4741 enhanced cell growth under various stress conditions. Meanwhile, ethanol productivity was also improved by overexpression of the three ADE genes under stress conditions, among which the highest improvement attained 158.39% by ADE17 overexpression in the presence of inhibitor mixtures derived from lignocellulosic biomass. Elevated levels of adenine-nucleotide pool “AXP” ([ATP] + [ADP] + [AMP]) and ATP content were observed by overexpression of ADE17, both under control condition and under acetic acid stress, and is consistent with the better growth of the recombinant yeast strain. The global intracellular amino acid profiles were also changed by overexpression of the ADE genes. Among the changed amino acids, significant increase of the stress protectant γ-aminobutyric acid (GABA) was revealed by overexpression of the ADE genes under acetic acid stress, suggesting that overexpression of the ADE genes exerts control on both purine biosynthesis and amino acid biosynthesis to protect yeast cells against the stress. Conclusion We proved that the de novo purine biosynthesis genes are useful targets for metabolic engineering of yeast stress tolerance. The engineered strains developed in this study with improved tolerance against multiple inhibitors can be employed for efficient lignocellulosic biorefinery to produce biofuels and biochemicals. Electronic supplementary material The online version of this article (10.1186/s13068-019-1456-1) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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