Nasal Epithelial Cells Can Act as a Physiological Surrogate for Paediatric Asthma Studies

Autor: Liam G Heaney, Michael E. McBrien, Michael D. Shields, Surendran Thavagnanam, Grzegorz Skibinski, JC Parker
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Chemokine
Pathology
Pulmonology
medicine.medical_treatment
Cellular differentiation
lcsh:Medicine
Stimulation
Biochemistry
Pediatrics
Molecular Cell Biology
Electric Impedance
lcsh:Science
Child
Multidisciplinary
biology
Cell Differentiation
respiratory system
Immunohistochemistry
Cytokine
medicine.anatomical_structure
Cytochemistry
Cytokines
Medicine
Cellular Types
Immunocytochemistry
Research Article
Cell physiology
medicine.medical_specialty
Pediatric Pulmonology
Enzyme-Linked Immunosorbent Assay
Respiratory Mucosa
Real-Time Polymerase Chain Reaction
Cell Growth
children
medicine
Humans
cell physiology
Biology
Secretion
Cell Proliferation
lcsh:R
Epithelial Cells
asthma
Molecular Development
Epithelium
cytokines
epithelial cells
Asthma
cell differentiation
Nasal Mucosa
Immunology
biology.protein
lcsh:Q
epithelium
Biomarkers
Developmental Biology
Zdroj: PLoS ONE
Thavagnanam, S, Parker, J C, McBrien, M E, Skibinski, G, Shields, M D & Heaney, L G 2014, ' Nasal Epithelial Cells Can Act as a Physiological Surrogate for Paediatric Asthma Studies ', PLoS ONE, vol. 9, no. 1, e85802 . https://doi.org/10.1371/journal.pone.0085802
PLoS ONE, Vol 9, Iss 1, p e85802 (2014)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0085802
Popis: Introduction: Differentiated paediatric epithelial cells can be used to study the role of epithelial cells in asthma. Nasal epithelial cells are easier to obtain and may act as a surrogate for bronchial epithelium in asthma studies. We assessed the suitability of nasal epithelium from asthmatic children to be a surrogate for bronchial epithelium using air-liquid interface cultures.Methods: Paired nasal and bronchial epithelial cells from asthmatic children (n = 9) were differentiated for 28 days under unstimulated and IL-13-stimulated conditions. Morphological and physiological markers were analysed using immunocytochemistry, transepithelial-electrical-resistance, Quantitative Real-time-PCR, ELISA and multiplex cytokine/chemokine analysis.Results: Physiologically, nasal epithelial cells from asthmatic children exhibit similar cytokine responses to stimulation with IL-13 compared with paired bronchial epithelial cells. Morphologically however, nasal epithelial cells differed significantly from bronchial epithelial cells from asthmatic patients under unstimulated and IL-13-stimulated conditions. Nasal epithelial cells exhibited lower proliferation/differentiation rates and lower percentages of goblet and ciliated cells when unstimulated, while exhibiting a diminished and varied response to IL-13.Conclusions: We conclude that morphologically, nasal epithelial cells would not be a suitable surrogate due to a significantly lower rate of proliferation and differentiation of goblet and ciliated cells. Physiologically, nasal epithelial cells respond similarly to exogenous stimulation with IL-13 in cytokine production and could be used as a physiological surrogate in the event that bronchial epithelial cells are not available.
Databáze: OpenAIRE
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