Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box
| Autor: | M. Zacheis, Joel Schiffenbauer, S.L Woulfe, Benjamin D. Schwartz, D.K Didier |
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| Rok vydání: | 1988 |
| Předmět: |
Molecular Sequence Data
CAAT box Biology DNA-binding protein Complementary DNA Amino Acid Sequence RNA Messenger Histone octamer Promoter Regions Genetic Peptide sequence HLA-DR Antigen Multidisciplinary Base Sequence Nuclear Proteins DNA HLA-DR Antigens Lambda phage Y box binding protein 1 biology.organism_classification Molecular biology DNA-Binding Proteins Molecular Weight NFI Transcription Factors CCAAT-Enhancer-Binding Proteins Y-Box-Binding Protein 1 Transcription Factors Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 85:7322-7326 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.85.19.7322 |
| Popis: | The expression of HLA class II genes is regulated by a series of cis-acting elements and trans-acting factors. Several cis-acting elements have been identified and have been termed the Z box, X box, Y box, octamer, and "TATA" box. The Y box contains an inverted CCAAT box. By probing a phage lambda gt11 library with double-stranded oligonucleotides, we have directly isolated a cDNA encoding a Y box-binding protein designated YB-1. YB-1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box. It has a Mr of 35,414, contains 18% basic residues, and contains putative nuclear localization signals. An inverse correlation of YB-1 and HLA-DR beta chain mRNA levels suggests that YB-1 is a negative regulatory factor. |
| Databáze: | OpenAIRE |
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