Assessment of the anti-rheumatoid arthritis activity of Gastrodia elata (tian-ma) and Radix aconitic lateralis preparata (fu-zi) via network pharmacology and untargeted metabolomics analyses
Autor: | Wei-Jun Ding, Di-Hong Gong, Jie Yang, Wei-Hong Li, Yu Zhang, Wei-Yi Yao, Bu-Fa Guo, Qi-Lun Peng |
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Rok vydání: | 2020 |
Předmět: |
Male
Linoleic acid Pharmacology Arthritis Rheumatoid Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Metabolomics Rheumatology Tandem Mass Spectrometry Metabolome Medicine Animals Humans Radix 030212 general & internal medicine Daidzin 030203 arthritis & rheumatology Aconitum Arachidonic Acid Gastrodia biology business.industry Plant Extracts Daidzein Membrane Proteins DNA biology.organism_classification Gastrodia elata Rats Disease Models Animal chemistry Gene Expression Regulation Cyclooxygenase 2 Isoflavonoid biosynthesis Cyclooxygenase 1 business Chromatography Liquid Drugs Chinese Herbal |
Zdroj: | International journal of rheumatic diseasesREFERENCES. 24(3) |
ISSN: | 1756-185X |
Popis: | Aim Gastrodia elata and Radix aconiti lateralis preparrata are respectively named as Tian-Ma and Fu-Zi (TF) in Chinese. We explored the active components against rheumatoid arthritis (RA) from an extensively used couplet of Chinese herbs, Gastrodia elata and Radix aconiti lateralis preparata (TF) via untargeted metabolomics and network pharmacological approaches. Methods Water extracts of TF were mixed at ratios 1:1, 3:2 and 2:3 (w/w). Ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) was then utilized as metabolomics screening. Human Metabolome (http://www.hmdb.ca/) and Lipidmaps (http://www.lipidmaps.org/) databases were used to annotate detected compounds. Further identification of vital genes and important pathways associated with the anti-RA properties of the TF preparations was done via network pharmacology, and verified by real-time quantitative polymerase chain reaction (RT-qPCR). Results Four key compounds involved in unsaturated fatty acid biosynthesis and isoflavonoid biosynthesis were identified through metabolomics analyses. Three key components of TF associated with anti-RA activity were linoleic acid, daidzein, and daidzin. Results of RT-qPCR revealed that all 3 tested TF couplets (1:1, 3:2, and 2:3) markedly suppressed the transcription of PTGS2. These results were consistent with our network pharmacological predictions. Conclusions The anti-RA properties of Tian-Ma and Fu-Zi are associated with the inhibition of arachidonic acid metabolism pathway. |
Databáze: | OpenAIRE |
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