Siroheme biosynthesis in Saccharomyces cerevisiae requires the products of both the MET1 and MET8 genes
| Autor: | Jørgen Hansen, H. Cherest, Marianne Muldbjerg, Yolande Surdin-Kerjan |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Molecular Sequence Data Saccharomyces cerevisiae Biophysics Heme Siroheme biosynthesis Biochemistry Cofactor Sulfite reductase Fungal Proteins chemistry.chemical_compound Uroporphyrinogen Structural Biology Siroheme Genetics Amino Acid Sequence Molecular Biology Gene Sequence Homology Amino Acid biology Methyltransferases Cell Biology biology.organism_classification Yeast Complementation Open reading frame Phenotype chemistry biology.protein Ferrochelatase |
| Zdroj: | FEBS Letters. 401:20-24 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(96)01423-8 |
| Popis: | Siroheme is a uroporphyrinogen III-derivative used by sulfite reductase as a prosthetic group. We investigated in Saccharomyces cerevisiae the possible involvement in siroheme biosynthesis of three genes, MET1, MET8 and MET20. The MET1 gene from S. cerevisiae was cloned and shown to be the same gene as MET20. Sequence similitudes as well as complementation studies indicate that Met1p and Met8p are both involved in siroheme biosynthesis. In addition, we show formally that S. cerevisiae does not need vitamin B12 for growth. |
| Databáze: | OpenAIRE |
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