Solute Concentration Affects Bradykinin-Mediated Increases in Renal Prostaglandin E2*
| Autor: | Terry V. Zenser, Elliot S. Davis, Neville S. Rapp, Bernard B. Davis |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Male
medicine.medical_specialty Bradykinin Stimulation In Vitro Techniques Tritium Dinoprostone chemistry.chemical_compound Endocrinology Internal medicine medicine Animals Urea Prostaglandin E2 Kidney Medulla Kidney Chemistry Prostaglandins E Osmolar Concentration Solutions Kinetics medicine.anatomical_structure Tonicity Arachidonic acid Rabbits Mannitol medicine.drug |
| Zdroj: | Endocrinology. 109:1927-1932 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-109-6-1927 |
| Popis: | The effects of solute concentration on the bradykinin-mediated increase in inner medullary slice prostaglandin E2 (PGE2) synthesis were investigated. PG content was determined by specific RIA. Bradykinin stimulation was prevented by the addition of the following solutes to Krebs buffer: 1.0 M urea, 0.5 or 1.0 M NaCl, 0.5 or 1.0 M mannitol, 1.0 M urea plus 0.5 M NaCl, or 1.0 M mannitol plus 0.5 M NaCl. By contrast, basal PGE2 synthesis was increased by 1.0 M mannitol or by 1.0 M mannitol plus 0.5 M NaCl, but decreased by 1.0 M urea. Urea elicited a concentration-dependent, reversible inhibition of bradykinin stimulation, with 0.01 M urea being the lowest effective concentration. By contrast, basal PGE2 synthesis was only reduced at a urea concentration greater than 0.6 M. Arachidonic acid-mediated increases in both PGE2 and PGF2 alpha synthesis were not prevented by 1.0 M urea. The latter suggests that neither PG endoperoxide synthetase nor PG endoperoxide E isomerase are inhibited by urea. The data indicate that different hypertonic solutions have different effects on basal PG production, but all inhibit bradykinin stimulation. |
| Databáze: | OpenAIRE |
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