Diethylstilbestrol increases the density of prolactin cells in male mouse pituitary by inducing proliferation of prolactin cells and transdifferentiation of gonadotropic cells
| Autor: | Shin-ichi Izumi, Satoshi Inoue, Yasuyoshi Ouchi, Keiko Shukuwa, Kuniaki Ejima, Masami Muramatsu, Takashi Kitaoka, Yoshitaka Hishikawa, Takehiko Koji |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Histology Blotting Western Diethylstilbestrol Estrogen receptor Thyrotropin Mice Internal medicine medicine Animals Estrogen Receptor beta Molecular Biology Cell Proliferation Mice Knockout Mice Inbred ICR Chemistry Transdifferentiation Estrogen Receptor alpha Cell Differentiation Cell Biology Luteinizing Hormone Immunohistochemistry Prolactin Blot Medical Laboratory Technology Endocrinology Ki-67 Antigen Growth Hormone Pituitary Gland Knockout mouse Electrophoresis Polyacrylamide Gel Follicle Stimulating Hormone Luteinizing hormone DNA Probes Transcription Factor Pit-1 hormones hormone substitutes and hormone antagonists Gonadotropins Hormone medicine.drug |
| Zdroj: | Histochemistry and cell biology. 126(1) |
| ISSN: | 0948-6143 |
| Popis: | Diethylstilbestrol (DES) has been implicated in mammalian abnormalities. We examined the effects of DES on follicle-stimulating hormone (FSH), luteinizing hormone (LH), and prolactin (PRL) cells in the pituitaries of male mice treated with various doses of DES for 20 days. DES reduced the density of FSH and LH cells in a dose-dependent manner, but increased that of PRL cells. When the expression of estrogen receptor (ER) alpha and beta was assessed, an induction of ERbeta by DES was found predominantly in PRL cells. However, since these effects were abolished in ERalpha knockout mice, DES appears to act primarily through ERalpha. When the expression of Ki-67 and Pit-1 in PRL cells was examined at various time-points after DES treatment, some PRL cells became Ki-67 positive at 10-15 days, and Pit-1-positive cells were increased at 5-15 days. Furthermore, some FSH and LH cells became Pit-1 positive, and co-localized with PRL at 5-10 days. Our results indicate that DES increases PRL cells by inducing proliferation of PRL cells and transdifferentiation of FSH/LH cells to PRL cells. |
| Databáze: | OpenAIRE |
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