Pentamethylquercetin induces adipose browning and exerts beneficial effects in 3T3-L1 adipocytes and high-fat diet-fed mice
| Autor: | Hui Liu, Man-Wen Jin, Lei Chen, Ji-zhong Shen, Yi Han, Ting He, Jian-Zhao Wu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Science Mice Obese Adipose tissue White adipose tissue Biology Carbohydrate metabolism Diet High-Fat Article Mice 03 medical and health sciences 3T3-L1 Cells Internal medicine Brown adipose tissue Adipocytes medicine Animals Obesity Uncoupling Protein 1 Cell Proliferation Multidisciplinary Gene Expression Profiling Cell Differentiation 3T3-L1 Lipid metabolism Lipid Metabolism Thermogenin Glucose Treatment Outcome 030104 developmental biology medicine.anatomical_structure Endocrinology Gene Expression Regulation Mitochondrial biogenesis Medicine Quercetin |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-01206-4 |
| Popis: | Browning white adipocytes may be a new target in anti-obesity therapy. Pentamethylquercetin (PMQ) has been shown to have anti-obesity effects in monosodium glutamate-induced obese mice. Here, we aimed to study the anti-obesity effects of PMQ in vitro and in vivo and to determine if adipose browning is involved in the mechanism underlying the anti-obesity effects of PMQ. We evaluated the effects of PMQ on cell proliferation, cell differentiation, glucose consumption, cellular lipid metabolism, and related brown gene expression in 3T3-L1 adipocytes. We also investigated the effects of PMQ in a mouse model of high-fat diet (HFD)-induced obesity. Our results demonstrated that PMQ increased the consumption of glucose, inhibited the accumulation of cellular triglycerides (TGs), and induced the expression of brown adipocyte-specific genes, such as uncoupling protein 1 (UCP-1), during the early stage of differentiation in 3T3-L1 adipocytes. In HFD mice, PMQ treatment reduced waist circumference, LEE index, white adipose tissue (WAT) weight and white adipocyte size and increased brown adipose tissue (BAT) weight. Moreover, PMQ treatment induced mitochondrial biogenesis and upregulated UCP-1 expression in WAT. These findings suggest that PMQ may induce browning of adipose tissue, a phenomenon that is at least partly related to its anti-obesity effects. |
| Databáze: | OpenAIRE |
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