Development of Contact Lens-Shaped Crosslinked Amniotic Membranes for Sutureless Fixation in the Treatment of Ocular Surface Diseases
| Autor: | Donghee Yoon, Dong Sung Kim, Hyeonjun Hong, Han Sang Park, Soo-Jin Yi, Man-Il Huh, Hong Kyun Kim |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Biocompatibility Contact Lenses Biomedical Engineering macromolecular substances Article Corneal Diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Tensile Strength Ultimate tensile strength dialdehyde starch medicine Humans Dialdehyde starch crosslinking Amnion Fixation (histology) amniotic membrane Chemistry sutureless fixation technology industry and agriculture Contact lens Ophthalmology 030104 developmental biology Membrane medicine.anatomical_structure Glutaral 030221 ophthalmology & optometry Glutaraldehyde Biomedical engineering |
| Zdroj: | Translational Vision Science & Technology |
| ISSN: | 2164-2591 |
| Popis: | Purpose To develop a new method of manufacturing contact lens-shaped crosslinked amniotic membranes (AMs) using glutaraldehyde (GA) and dialdehyde starch (DAS) as crosslinking agents. Methods Amniotic membranes were placed on a curved plastic mold and crosslinked with either 4.5% DAS or 1% GA, after which their physical properties and biological safety were evaluated. Results The tensile strength of the GA- and DAS-crosslinked samples was much increased compared with that of normal AMs. Neither crosslinking process affected AM transparency. Although the GA-crosslinked AM showed better enzymatic resistance, its physiological structure was severely damaged after the crosslinking process. On the other hand, compared with the GA-crosslinked AM, the DAS-crosslinked AM showed higher growth factor concentrations and better biocompatibility, similar to normal AMs. In addition, the DAS-crosslinked AM was effective in the recovery of corneal epithelial wounds and was well maintained over 3 days without decentration or degradation on the ocular surface in human subjects. Conclusions Contact lens-shaped AMs were successfully prepared with crosslinking agents. Crosslinking with DAS did not affect the structural properties or biological activity of the AMs, and the improved mechanical properties helped the AM to maintain its curved shape. This crosslinking method allowed us to transplant AMs into patients' eyes without sutures. Translational relevance Sutureless fixation of contact lens-shaped AMs would be very convenient and safe for the treatment of corneal surface disease. |
| Databáze: | OpenAIRE |
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