Comparative Study of Glyceryl Behenate or Polyoxyethylene 40 Stearate-Based Lipid Carriers for Trans-Resveratrol Delivery: Development, Characterization and Evaluation of the In Vitro Tyrosinase Inhibition
| Autor: | Roberta Balansin Rigon, Mariana Rillo Sato, Marlus Chorilli, Karen Cristina dos Santos, Josimar O. Eloy, Naiara Fachinetti |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Tyrosinase
Pharmaceutical Science 02 engineering and technology Aquatic Science tyrosinase Cell Line Polyethylene Glycols 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Stearate Drug Discovery PEG ratio Stilbenes Animals Enzyme Inhibitors Particle Size Cytotoxicity Ecology Evolution Behavior and Systematics Drug Carriers Chromatography Ecology Chemistry Monophenol Monooxygenase Fatty Acids General Medicine 021001 nanoscience & nanotechnology trans-resveratrol Nanostructures polyoxyethylene 40 stearate glyceryl behenate Resveratrol 030220 oncology & carcinogenesis nanostructured lipids carriers Glyceryl behenate Nanocarriers 0210 nano-technology Drug carrier Agronomy and Crop Science |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2018-12-11T17:19:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-04-01 Trans-resveratrol (RSV) is a natural compound with several properties, such as the ability to inhibit the tyrosinase enzyme, with potential application as a skin-lightning agent and for the treatment of skin disorders associated with hyperpigmentation and melanogenesis. However, the drug faces several drawbacks which altogether limit its therapeutic application. Thus, drug loading into nanocarriers emerge as an alternative to circumvent these problems. Herein, nanostructured lipid carriers (NLCs) have been employed for RSV encapsulation, with comparison of two different lipids, glyceryl behenate (more hydrophobic), and polyoxyethylene 40 (PEG 40) stearate. PEG 40 stearate-containing NLCs presented smaller particle size and polydispersity compared with glyceryl behenate, attributed to better emulsification and nanoparticle formation, resulting in higher RSV encapsulation efficiency. Drug was loaded in both carriers as a molecular dispersion. Furthermore, the formulations had very low RSV release, which occurred due to the crystallinity degree of lipid matrix, in accordance with the DSC data. Moreover, RSV cytotoxicity against L-929 cells was not increased when loaded into nanocarriers. Interestingly, RSV-loaded formulation prepared with PEG-40 stearate resulted on greater tyrosinase inhibition than RSV solution and formulation containing glyceryl behenate, equivalent to 1.31 and 1.83 times higher, respectively, demonstrating that the incorporation of RSV into NLC allowed an enhanced tyrosinase inhibitory activity. Overall, the results obtained herein evidence potential for future in vivo evaluation of RSV-loaded NLCs. School of Pharmaceutical Sciences Campus Araraquara Department of Drugs and Medicines UNESP-São Paulo State University, Rodovia Araraquara-Jau, km 1, Araraquara School of Pharmaceutical Sciences Campus Araraquara Department of Drugs and Medicines UNESP-São Paulo State University, Rodovia Araraquara-Jau, km 1, Araraquara |
| Databáze: | OpenAIRE |
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