FoxO and Stress Responses in the Cnidarian Hydra vulgaris
| Autor: | Emily Marcinkevicius, Alexander G. Theofiles, Rebecca L. Holler, Robert Steele, Daniel E. Martínez, Diane M. Bridge |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
MAP Kinase Kinase 4
lcsh:Medicine Evolutionary Biology/Developmental Evolution stem-cells 0302 clinical medicine oxidative stress lcsh:Science Caenorhabditis elegans Phylogeny Genetics 0303 health sciences Multidisciplinary biology n-terminal kinase Life Sciences Forkhead Transcription Factors Developmental Biology/Stem Cells Hydra vulgaris forkhead transcription factor multiple sequence alignment Lernaean Hydra Stem cell Developmental Biology/Developmental Evolution hormones hormone substitutes and hormone antagonists Research Article animal structures Green Fluorescent Proteins Molecular Sequence Data 03 medical and health sciences Cnidaria Animals Amino Acid Sequence Heat shock Protein kinase B Transcription factor 030304 developmental biology life-span Cell Nucleus Sequence Homology Amino Acid lcsh:R fungi coral montastraea-faveolata Developmental Biology/Aging biology.organism_classification gene-expression caenorhabditis-elegans cell-cycle Multipotent Stem Cell lcsh:Q Proto-Oncogene Proteins c-akt 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE PLoS ONE, Vol 5, Iss 7, p e11686 (2010) Bridge, Diane; Theofiles, Alexander G.; Holler, Rebecca L.; Marcinkevicius, Emily; Steele, Robert E.; & Martinez, Daniel E.(2010). FoxO and Stress Responses in the Cnidarian Hydra vulgaris. PLoS ONE, 5(7). UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/77x7895p |
| ISSN: | 1932-6203 |
| Popis: | Background In the face of changing environmental conditions, the mechanisms underlying stress responses in diverse organisms are of increasing interest. In vertebrates, Drosophila, and Caenorhabditis elegans, FoxO transcription factors mediate cellular responses to stress, including oxidative stress and dietary restriction. Although FoxO genes have been identified in early-arising animal lineages including sponges and cnidarians, little is known about their roles in these organisms. Methods/Principal Findings We have examined the regulation of FoxO activity in members of the well-studied cnidarian genus Hydra. We find that Hydra FoxO is expressed at high levels in cells of the interstitial lineage, a cell lineage that includes multipotent stem cells that give rise to neurons, stinging cells, secretory cells and gametes. Using transgenic Hydra that express a FoxO-GFP fusion protein in cells of the interstitial lineage, we have determined that heat shock causes localization of the fusion protein to the nucleus. Our results also provide evidence that, as in bilaterian animals, Hydra FoxO activity is regulated by both Akt and JNK kinases. Conclusions These findings imply that basic mechanisms of FoxO regulation arose before the evolution of bilaterians and raise the possibility that FoxO is involved in stress responses of other cnidarian species, including corals. |
| Databáze: | OpenAIRE |
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