Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised inE. coli
| Autor: | Michael A. Boss, Kenten Jh, Clive Ross Wood, Emtage Js |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Immunoglobulin lambda-Chains
Biology medicine.disease_cause Immunoglobulin light chain Antibodies Mice Escherichia coli Genetics medicine Animals Cloning Molecular Binding site Base Sequence Immunoglobulin mu-Chains DNA Restriction Enzymes Molecular biology Ribosomal binding site Kinetics Genes Biochemistry biology.protein Immunoglobulin heavy chain Immunoglobulin Light Chains Antibody Immunoglobulin Heavy Chains Haptens Plasmids |
| Zdroj: | Nucleic Acids Research. 12:3791-3806 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/12.9.3791 |
| Popis: | Genes for a murine mu heavy chain and a lambda light chain immunoglobulin have been inserted into bacterial expression plasmids containing the Escherichia coli trp promoter and ribosome binding site. Induction of transcription from the trp promoter results in accumulation of both light and heavy chain polypeptides in appropriate host strains. Both proteins were found as insoluble products. Following extraction and purification of the immunoglobulin containing fractions, antigen binding activity was recovered. The activity demonstrates essentially the same properties as the antibody from the hybridoma from which the genes were cloned. |
| Databáze: | OpenAIRE |
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