Centrifugal LabTube platform for fully automated DNA purification and LAMP amplification based on an integrated, low-cost heating system
| Autor: | Roland Zengerle, Thomas Nesch, Melanie Hoehl, Alexander H. Slocum, Nils Paust, Felix von Stetten, Arne Dannenberg, Juergen Steigert, Michael Weißert |
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| Přispěvatelé: | Harvard University--MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology. Department of Mechanical Engineering, Slocum, Alexander H., Hoehl, Melanie M. |
| Rok vydání: | 2014 |
| Předmět: |
Materials science
Biomedical Engineering Analytical chemistry Centrifugation Polymerase Chain Reaction law.invention Heating Automation Electric Power Supplies law Laboratory centrifuge Disposable Equipment Process engineering Molecular Biology Shiga-Toxigenic Escherichia coli Elution business.industry Battery (vacuum tube) DNA DNA extraction Systems Integration Microcontroller Heating system Fully automated Resistor business Food Analysis |
| Zdroj: | Prof. Slocum via Angie Locknar |
| ISSN: | 1572-8781 1387-2176 |
| DOI: | 10.1007/s10544-014-9841-9 |
| Popis: | This paper introduces a disposable battery-driven heating system for loop-mediated isothermal DNA amplification (LAMP) inside a centrifugally-driven DNA purification platform (LabTube). We demonstrate LabTube-based fully automated DNA purification of as low as 100 cell-equivalents of verotoxin-producing Escherichia coli (VTEC) in water, milk and apple juice in a laboratory centrifuge, followed by integrated and automated LAMP amplification with a reduction of hands-on time from 45 to 1 min. The heating system consists of two parallel SMD thick film resistors and a NTC as heating and temperature sensing elements. They are driven by a 3 V battery and controlled by a microcontroller. The LAMP reagents are stored in the elution chamber and the amplification starts immediately after the eluate is purged into the chamber. The LabTube, including a microcontroller-based heating system, demonstrates contamination-free and automated sample-to-answer nucleic acid testing within a laboratory centrifuge. The heating system can be easily parallelized within one LabTube and it is deployable for a variety of heating and electrical applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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