Impact of cellular health conditions on the protein folding state in mammalian cells
| Autor: | Masaomi Ikari, Takanori Kigawa, Hajime Kamoshida, Kohsuke Inomata, Yutaka Ito |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Protein Folding Chemistry Adenylate Kinase Metals and Alloys General Chemistry Catalysis Surfaces Coatings and Films Electronic Optical and Magnetic Materials 03 medical and health sciences 030104 developmental biology Biochemistry Materials Chemistry Ceramics and Composites Humans Protein folding Nuclear Magnetic Resonance Biomolecular HeLa Cells |
| Zdroj: | Chemical communications (Cambridge, England). 53(81) |
| ISSN: | 1364-548X |
| Popis: | By using in-cell NMR experiments, we have demonstrated that the protein folding state in cells is significantly influenced by the cellular health conditions. hAK1 was denatured in cells under stressful culture conditions, while it remained functional and properly folded in cells continuously supplied with a fresh medium. |
| Databáze: | OpenAIRE |
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