Nano-Sized Sunflower Polycations As Effective Gene Transfer Vehicles
| Autor: | David J. Peeler, James Kevin Y Tan, Don O. Maris, Drew L. Sellers, Philip J. Horner, Hua Wei, Suzie H. Pun, Yilong Cheng |
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| Rok vydání: | 2016 |
| Předmět: |
Magnetic Resonance Spectroscopy
animal structures Materials science 02 engineering and technology Gene delivery 010402 general chemistry Methacrylate 01 natural sciences Article Biomaterials Nucleic Acids Spectroscopy Fourier Transform Infrared Polyamines General Materials Science Cytotoxicity chemistry.chemical_classification Molecular Structure Atom-transfer radical-polymerization fungi Gene Transfer Techniques General Chemistry Polymer Transfection 021001 nanoscience & nanotechnology Polyelectrolytes Sunflower 0104 chemical sciences Nylons chemistry Biochemistry Nucleic acid Biophysics Methacrylates 0210 nano-technology Biotechnology |
| Zdroj: | Small. 12:2750-2758 |
| ISSN: | 1613-6810 |
| DOI: | 10.1002/smll.201502930 |
| Popis: | The architecture of polycations plays an important role in both gene transfection efficiency and cytotoxicity. In this work, a new polymer, sunflower poly(2-dimethyl amino)ethyl methacrylate) (pDMAEMA) were prepared by atom transfer radical polymerization (ATRP) and employed as nucleic acid carriers compared to linear pDMAEMA homopolymer and comb pDMAEMA. The sunflower pDMAEMAs showed higher IC50, greater buffering capacity, and stronger binding capacity towards plasmid DNA than their linear and comb counterparts. In vitro transfection studies demonstrated that sunflower pDMAEMAs exhibited high transfection efficiency as well as relatively low cytotoxicity in complete growth medium. In vivo gene delivery by intraventricular injection to the brain showed that sunflower polymer delivers plasmid DNA more effectively than comb polymer. This study provides a new insight into the relationship between polymeric architecture and gene delivery capability, and as well as a useful means to design potent vectors for successful gene delivery. |
| Databáze: | OpenAIRE |
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