Determination of dissolved nickel in natural waters using a rapid microplate fluorescence assay method

Autor: Fabien Robert-Peillard, El Mountassir El Mouchtari, Damien Bonne, Stéphane Humbel, Jean-Luc Boudenne, Bruno Coulomb
Přispěvatelé: Laboratoire Chimie de l'environnement (LCE), Aix Marseille Université (AMU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires de Marseille (ISM2), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), ANR-18-CE04-0005,SMART-3D,Dispositif analytique innovant imprimé en 3D pour l'analyse des métaux lourds avec détection par smartphone(2018)
Rok vydání: 2021
Předmět:
Zdroj: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy [1994-...]
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy [1994-..], 2022, 275, pp.121170. ⟨10.1016/j.saa.2022.121170⟩
ISSN: 1873-3557
1386-1425
Popis: International audience; A new microplate analytical procedure is described for the determination of nickel (Ni2+) ions in natural water samples. A lophine analogue fluorescent sensor was synthesized and a spectral study showed a selective fluorescence quenching effect of chemical sensor by Ni2+ under optimized conditions. Density functional theory (DFT) calculations confirmed the formation of a Ni(II)L3 complex obtained by the Job plot. The calculations showed that the fluorescence emission peak of L collapses due to the distortion of L in the complex. The simple and fast microplate procedure allowed us to quantify Ni2+ with a linear response from 1.6 to 40 µg L-1 and a quantification limit of 5.4 µg L-1 without the need of a preconcentration step. The optimized procedure using high-throughput microplate assay has been applied for the determination of Ni 2+ in natural water samples with good analytical performances.
Databáze: OpenAIRE
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