Anti-vascular inflammatory effects of pentacyclic triterpenoids from Astilbe rivularis in vitro and in vivo
| Autor: | Jiwoo Chung, Hyejin Kang, Jongdoo Kim, Wei Zhou, Sae-Kwang Ku, Jong-Sup Bae, Sang Chan Kim, MinKyun Na |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Male Leukocyte migration Lipopolysaccharide Neutrophils Anti-Inflammatory Agents Inflammation Punctures Pharmacology Biology Toxicology Protective Agents Umbilical vein Permeability Extracellular matrix 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Cell Movement Transforming Growth Factor beta Sepsis medicine Cell Adhesion Human Umbilical Vein Endothelial Cells Animals Humans Cecum Ligation Lung Extracellular Matrix Proteins Cell adhesion molecule Interleukin-6 Tumor Necrosis Factor-alpha Saxifragaceae NF-kappa B General Medicine In vitro Mice Inbred C57BL 030104 developmental biology Biochemistry chemistry 030220 oncology & carcinogenesis medicine.symptom Pentacyclic Triterpenes Cell Adhesion Molecules |
| Zdroj: | Chemico-biological interactions. 261 |
| ISSN: | 1872-7786 |
| Popis: | Sepsis is a systemic inflammatory condition resulting from bacterial infections. It is associated with high mortality rates, and its therapeutic options are limited. Transforming growth factor β induced protein (TGFBIp) is an extracellular matrix protein that functions as a mediator of experimental sepsis. C-27-carboxylated pentacyclic triterpenoids are specifically found in species of the genus Astilbe, and show several biological effects. Given the anti-inflammatory effects of pentacyclic triterpenoids, we investigated the effects of 3β-trans-p-coumaroyloxy-olean-12-en-27-oic acid (1) and 6β-hydroxy-3-oxoolean-12-en-27-oic acid (2) on TGFBIp-mediated vascular inflammatory responses. The anti-inflammatory activities of compounds 1 and 2 were determined by measuring the permeability, leukocyte adhesion and migration, and activation of pro-inflammatory proteins in TGFBIp-activated human umbilical vein endothelial cells (HUVECs) and mice. We found that compounds 1 and 2 inhibited lipopolysaccharide (LPS)-induced TGFBIp secretion, TGFBIp-induced barrier disruption, expression of cell adhesion molecules (CAMs), and the adhesion/transendothelial migration of the neutrophils to the human endothelial cells. Compounds 1 and 2 also suppressed TGFBIp-induced hyperpermeability and leukocyte migration in vivo. These results suggested that C-27-carboxylated pentacyclic triterpenoids 1 and 2 have anti-inflammatory functions by inhibiting hyperpermeability, CAM expression, and leukocyte adhesion/migration. Therefore, these compounds can be considered as a potential therapy for vascular inflammatory diseases. |
| Databáze: | OpenAIRE |
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