Development of a temperature-responsive agarose-based ion-exchange chromatographic resin
| Autor: | W. Roy Jackson, Milton Thomas William Hearn, Kirthi De Silva, Pankaj Maharjan, Brad W. Woonton |
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| Rok vydání: | 2008 |
| Předmět: |
Magnetic Resonance Spectroscopy
Ion chromatography Lactoglobulins Chemical Fractionation Sodium Chloride Biochemistry Lower critical solution temperature Analytical Chemistry chemistry.chemical_compound Adsorption Desorption Animals Acrylamides Chromatography Ion exchange biology Lactoferrin Chemistry Sepharose Organic Chemistry Cationic polymerization Temperature General Medicine Chromatography Ion Exchange Solutions Cross-Linking Reagents Models Chemical biology.protein Lactalbumin Solvents Agarose Cattle Ion Exchange Resins |
| Zdroj: | Journal of chromatography. A. 1216(50) |
| ISSN: | 1873-3778 |
| Popis: | A temperature-responsive ion-exchange resin (ItBA) has been prepared by grafting poly(N-isopropylacrylamide-co-acrylic acid-co-tert-butylacrylamide; ItBA) onto cross-linked agarose. A carboxymethylated ion exchanger (CM) of similar charge density was also prepared. Maximum adsorption capacities (B(max)) for lactoferrin at 20 degrees C and 50 degrees C were determined for both resins by batch adsorption procedures. Dynamic adsorption and desorption characteristics of the CM and ItBA with lactoferrin were established, as well as the ability of ItBA to selectively adsorb and desorb lactoferrin in the presence of other proteins. With the CM-agarose resin there was no significant difference between the B(max) values obtained at 20 degrees C and 50 degrees C. However, for the agarose-based ItBA resin the B(max) value at 50 degrees C was almost three times higher than the B(max) value at 20 degrees C. Dynamically, lactoferrin adsorbed to the ItBA packed column at 50 degrees C with a significant proportion of the adsorbed lactoferrin desorbed by reducing the temperature to 20 degrees C. In addition, anionic proteins did not adsorb to the ItBA packed column, and did not interfere with the dynamic adsorption/desorption behaviour of lactoferrin. These results indicate that this new temperature-responsive agarose-based ItBA resin has potential for the fractionation of whey proteins, with good selectivity for cationic proteins. |
| Databáze: | OpenAIRE |
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