Aedes aegypti on Madeira Island (Portugal): genetic variation of a recently introduced dengue vector
| Autor: | Melina Campos, Carine Spenassatto, João Pinto, Gonçalo Seixas, Carla A. Sousa, Matias Reyes-Lugo, M. T. Novo, Patrícia Salgueiro, Paulo Eduardo Martins Ribolla, Ana Clara Silva |
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| Přispěvatelé: | Instituto de Higiene e Medicina Tropical (IHMT), Centro de Malária e outras Doenças Tropicais (CMDT), Unidade de Parasitologia e Microbiologia Médicas (UPMM), Unidade de Ensino e Investigacao-Parasitologia Medica, Centro de Malaria e outras Doencas Tropicais, Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical Unidade de Parasitologia e Microbiologia Medicas, Instituto de Administracao da Saude e Assuntos Sociais Unidade de Engenharia Sanitaria Departamento de Promocao e Protecao da Saude, Universidade Estadual Paulista (Unesp), Universidade Estadual de Londrina (UEL) |
| Rok vydání: | 2013 |
| Předmět: |
knockdown resistance
lcsh:QR1-502 lcsh:Microbiology Disease Outbreaks Insecticide Resistance 0302 clinical medicine Aedes aegypti Aedes SDG 15 - Life on Land 0303 health sciences education.field_of_study biology Ecology mtDNA Articles 3. Good health Infectious Diseases Brazil Microbiology (medical) lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 030231 tropical medicine Population Zoology DNA Mitochondrial Electron Transport Complex IV 03 medical and health sciences SDG 3 - Good Health and Well-being Genetic variation parasitic diseases Genetics Animals Genetic variability education Ecology Evolution Behavior and Systematics 030304 developmental biology Genetic diversity Portugal fungi Knockdown resistance NADH Dehydrogenase biology.organism_classification Venezuela dengue Insect Vectors Haplotypes Insect Science Mutation Animal Distribution Founder effect |
| Zdroj: | Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz., Vol 108, Iss suppl 1, Pp 3-10 (2013) Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Memórias do Instituto Oswaldo Cruz, Volume: 108 Supplement 1, Pages: 10-3, Published: 2013 |
| ISSN: | 1678-8060 0074-0276 |
| Popis: | Made available in DSpace on 2014-09-30T18:18:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2013Bitstream added on 2014-10-01T14:04:14Z : No. of bitstreams: 1 S0074-02762013000900003.pdf: 529161 bytes, checksum: 81385c72bbd25c5d11a271d286f7317a (MD5) The increasing population of Aedes aegypti mosquitoes on Madeira Island (Portugal) resulted in the first autochthonous dengue outbreak, which occurred in October 2012. Our study establishes the first genetic evaluation based on the mitochondrial DNA (mtDNA) genes [cytochrome oxidase subunit I (COI) and NADH dehydrogenase subunit 4 (ND4)] and knockdown resistance ( kdr ) mutations exploring the colonisation history and the genetic diversity of this insular vector population. We included mosquito populations from Brazil and Venezuela in the analysis as putative geographic sources. The Ae. aegyptipopulation from Madeira showed extremely low mtDNA genetic variability, with a single haplotype for COI and ND4. We also detected the presence of two important kdr mutations and the quasi-fixation of one of these mutations (F1534C). These results are consistent with a unique recent founder event that occurred on the island of Ae. aegyptimosquitoes that carry kdr mutations associated with insecticide resistance. Finally, we also report the presence of the F1534C kdr mutation in the Brazil and Venezuela populations. To our knowledge, this is the first time this mutation has been found in South American Ae. aegypti mosquitoes. Given the present risk of Ae. aegypti re-invading continental Europe from Madeira and the recent dengue outbreaks on the island, this information is important to plan surveillance and control measures. Unidade de Ensino e Investigacao-Parasitologia Medica Centro de Malaria e outras Doencas Tropicais Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical Unidade de Parasitologia e Microbiologia Medicas Instituto de Administracao da Saude e Assuntos Sociais Unidade de Engenharia Sanitaria Departamento de Promocao e Protecao da Saude Universidade Estadual Paulista Instituto de Biociencias Departamento de Parasitologia Universidad Central de Venezuela Instituto de Medicina Tropical Seccion Entomologia Medica Universidade Estadual Paulista Instituto de Biociencias Departamento de Parasitologia |
| Databáze: | OpenAIRE |
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