A bis-indole/carbazole based C5-curcuminoid fluorescent probe with large Stokes shift for selective detection of biothiols and application to live cell imaging
| Autor: | Kasturi Chatterjee, Chiranjit Acharya, Parasuraman Jaisankar, Debkumar Nandi, Anushree Achari, Surajit Ghosh, Snehasikta Swarnakar, Sourav Chatterjee, Abhijit Saha, Pinaki Bhattacharjee |
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| Rok vydání: | 2019 |
| Předmět: |
Indoles
Cell Survival Carbazoles Color 010402 general chemistry Photochemistry 01 natural sciences Biochemistry Analytical Chemistry Cell Line chemistry.chemical_compound symbols.namesake Live cell imaging Diarylheptanoids Limit of Detection Stokes shift Electrochemistry Environmental Chemistry Humans Curcuminoid Sulfhydryl Compounds Spectroscopy Fluorescent Dyes 010405 organic chemistry Carbazole Optical Imaging Glutathione Fluorescence 0104 chemical sciences Spectrometry Fluorescence chemistry Acetylation symbols Cysteine |
| Zdroj: | The Analyst. 145(4) |
| ISSN: | 1364-5528 |
| Popis: | A series of heterocyclic C5-curcuminoids (bis(arylmethylidene)acetones) (PJ1-PJ6) having a large Stokes shift (Δλ = 104-173 nm) have been synthesized for the selective detection of cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) in living cells. The compounds were synthesized using a new methodology via deacetylation under microwave conditions. The photophysical properties of these compounds have been studied. Prominent colour changes from bright yellow to colourless in the presence of thiols were observed for PJ1. Live cell imaging has been employed with PJ1 for the utilization of the probe to detect homocysteine in A375 cells and apoptosis in AGS cells. |
| Databáze: | OpenAIRE |
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