Acetylcholine via Muscarinic Receptors Inhibits Histamine Release from Human Isolated Bronchi
| Autor: | Dirk Baumgärtner, Torsten Reinheimer, Karl-Dieter Höhle, Ignaz Wessler, Kurt Racké |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Atropine
Pulmonary and Respiratory Medicine Agonist Physostigmine medicine.medical_specialty Time Factors medicine.drug_class Bradykinin Bronchi Muscarinic Antagonists Muscarinic Agonists Critical Care and Intensive Care Medicine Histamine Release chemistry.chemical_compound Culture Techniques Internal medicine Muscarinic acetylcholine receptor medicine Oxotremorine Humans Drug Interactions Calcimycin Dose-Response Relationship Drug Ionophores business.industry Immunoglobulin E Receptors Muscarinic Acetylcholine Endocrinology chemistry Acetylcholinesterase inhibitor Depression Chemical Cholinesterase Inhibitors business Histamine medicine.drug |
| Zdroj: | American Journal of Respiratory and Critical Care Medicine. 156:389-395 |
| ISSN: | 1535-4970 1073-449X |
| Popis: | Human bronchi were incubated in organ baths to measure histamine release. The calcium ionophore A23187 (10 mumol/L; 1 min) stimulated histamine release by 148 +/- 28% (n = 11) above the prestimulation level but was ineffective in epithelium-denuded bronchi. Neither bradykinin (0.1 mumol/L) nor compound 48/80 (10 micrograms/ml) triggered the release of histamine from epithelium-intact bronchi. Acetylcholine did not affect spontaneous histamine release (about 2 nmol/g x 5 min) but inhibited A23187-evoked histamine release in an atropine-sensitive manner. Already a concentration as low as 0.1 nmol/L acetylcholine was effective, the maximal inhibition (by 89%) occurred at 100 nmol/L, whereas a concentration of 10 mumol/L acetylcholine was ineffective. Oxotremorine (1 nmol/L), a stable agonist at muscarinic receptors, suppressed stimulated histamine release completely. Physostigmine (0.1 mumol/L), an acetylcholinesterase inhibitor, reduced A23187-evoked histamine release by 58%. Antihuman IgE antibody stimulated histamine release by 127 +/- 30% (n = 6) above the prestimulation level. Acetylcholine (100 nmol/L) inhibited also the immunologically evoked histamine release by 70%. In conclusion, the present experiments provide a model to characterize mast cells that are localized in or close to the airway surface epithelium. Acetylcholine via muscarinic receptors strongly inhibits the releasability of these mucosal mast cells being among the first cells to interact with inhaled antigens and environmental agents. The inhibitory action of physostigmine indicates the involvement of endogenous, probably non-neuronal acetylcholine expressed in airway epithelial cells. |
| Databáze: | OpenAIRE |
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