Condensation of Ded1p Promotes a Translational Switch from Housekeeping to Stress Protein Production
| Autor: | Ulf-Peter Guenther, António Miguel de Jesus Domingues, Taraneh Zarin, Ceciel Jegers, Moritz Kreysing, Simon Alberti, Alan M. Moses, Lena Hersemann, Günter Kramer, Titus M. Franzmann, Marcus Jahnel, Christiane Iserman, Christine Desroches Altamirano, Matthias W. Hentze, Doris Richter, Anthony A. Hyman, Anatol Fritsch, Matthäus Mittasch, Ulrike Anne Friedrich |
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| Rok vydání: | 2020 |
| Předmět: |
Untranslated region
Saccharomyces cerevisiae Proteins Gene Expression Saccharomyces cerevisiae General Biochemistry Genetics and Molecular Biology DEAD-box RNA Helicases 03 medical and health sciences 0302 clinical medicine Gene Expression Regulation Fungal Gene expression Homologous chromosome Protein biosynthesis RNA Messenger Ribosome profiling Heat shock Heat-Shock Proteins 030304 developmental biology 0303 health sciences Genes Essential biology 3. Good health Heat stress Cell biology Protein Biosynthesis Chaperone (protein) biology.protein Ribosomes Heat-Shock Response 030217 neurology & neurosurgery |
| Zdroj: | Cell |
| ISSN: | 0092-8674 |
| Popis: | Summary Cells sense elevated temperatures and mount an adaptive heat shock response that involves changes in gene expression, but the underlying mechanisms, particularly on the level of translation, remain unknown. Here we report that, in budding yeast, the essential translation initiation factor Ded1p undergoes heat-induced phase separation into gel-like condensates. Using ribosome profiling and an in vitro translation assay, we reveal that condensate formation inactivates Ded1p and represses translation of housekeeping mRNAs while promoting translation of stress mRNAs. Testing a variant of Ded1p with altered phase behavior as well as Ded1p homologs from diverse species, we demonstrate that Ded1p condensation is adaptive and fine-tuned to the maximum growth temperature of the respective organism. We conclude that Ded1p condensation is an integral part of an extended heat shock response that selectively represses translation of housekeeping mRNAs to promote survival under conditions of severe heat stress. |
| Databáze: | OpenAIRE |
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