Annexin A1 Mimetic Peptide and Piperlongumine: Anti-Inflammatory Profiles in Endotoxin-Induced Uveitis
| Autor: | Marinônio Lopes Cornélio, Helena Ribeiro Souza, Caroline de Freitas Zanon, Sara de Souza Costa, Ana Paula Girol, Ícaro Putinhon Caruso, Sonia Maria Oliani |
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| Přispěvatelé: | University Center Padre Albino (UNIFIPA), Universidade Estadual Paulista (UNESP), Universidade de São Paulo (USP), União das Faculdades dos Grandes Lagos (Unilago) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Lipopolysaccharide Neutrophils medicine.drug_class QH301-705.5 inflammatory mediators Ac2-26 Anti-Inflammatory Agents Pharmacology Eye Models Biological Peripheral blood mononuclear cell Article Monocytes Anti-inflammatory Uveitis Eye inflammation chemistry.chemical_compound medicine Animals Cilia Natural bioactive extracts Rats Wistar Receptors Lipoxin Biology (General) FPR receptor Piperlongumine Annexin A1 Formyl peptide receptor biology lipopolysaccharide Dioxolanes General Medicine medicine.disease natural bioactive extracts Inflammatory mediators Endotoxins chemistry Cyclooxygenase 2 eye inflammation biology.protein Cyclooxygenase Inflammation Mediators Peptides |
| Zdroj: | Cells, Vol 10, Iss 3170, p 3170 (2021) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Cells Volume 10 Issue 11 |
| ISSN: | 2073-4409 |
| Popis: | Made available in DSpace on 2022-04-29T08:36:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-11-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Uveitis is one of the main causes of blindness worldwide, and therapeutic alternatives are worthy of study. We investigated the effects of piperlongumine (PL) and/or annexin A1 (AnxA1) mimetic peptide Ac2-26 on endotoxin-induced uveitis (EIU). Rats were inoculated with lipopolysac-charide (LPS) and intraperitoneally treated with Ac2-26 (200 µg), PL (200 and 400 µg), or Ac2-26 + PL after 15 min. Then, 24 h after LPS inoculation, leukocytes in aqueous humor, mononuclear cells, AnxA1, formyl peptide receptor (fpr)1, fpr2, and cyclooxygenase (COX)-2 were evaluated in the ocular tissues, along with inflammatory mediators in the blood and macerated supernatant. Decreased leukocyte influx, levels of inflammatory mediators, and COX-2 expression confirmed the anti-inflammatory actions of the peptide and pointed to the protective effects of PL at higher dosage. However, when PL and Ac2-26 were administered in combination, the inflammatory potential was lost. AnxA1 expression was elevated among groups treated with PL or Ac2-26 + PL but reduced after treatment with Ac2-26. Fpr2 expression was increased only in untreated EIU and Ac2-26 groups. The interaction between Ac2-26 and PL negatively affected the anti-inflammatory action of Ac2-26 or PL. We emphasize that the anti-inflammatory effects of PL can be used as a therapeutic strategy to protect against uveitis. Department of Physical and Morphological Sciences University Center Padre Albino (UNIFIPA) Department of Biology Institute of Biosciences Humanities and Exact Sciences (Ibilce) São Paulo State University (UNESP) Escola Paulista de Medicina (UNIFESP-EPM) Federal University of São Paulo Department of Phisics Institute of Biosciences Humanities and Exact Sciences (Ibilce) São Paulo State University (UNESP) Advanced Research Center in Medicine (CEPAM) União das Faculdades dos Grandes Lagos (Unilago) Department of Biology Institute of Biosciences Humanities and Exact Sciences (Ibilce) São Paulo State University (UNESP) Department of Phisics Institute of Biosciences Humanities and Exact Sciences (Ibilce) São Paulo State University (UNESP) FAPESP: 2015/03359-5 FAPESP: 2019/19949-7 CNPq: 404190/2016-2 |
| Databáze: | OpenAIRE |
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