The feasibility of detecting endometrial and ovarian cancer using DNA methylation biomarkers in cervical scrapings
| Autor: | Cheng Chang Chang, Hui Chen Wang, Yu Chih Chen, Mu Hsien Yu, Yu Chun Weng, Yu Ping Liao, Hung Cheng Lai |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
0301 basic medicine Uterine Cervical Neoplasms Cervix Uteri Sensitivity and Specificity law.invention Andrology 03 medical and health sciences 0302 clinical medicine law Biomarkers Tumor Humans Dna pools Medicine Early Detection of Cancer Polymerase chain reaction Aged Retrospective Studies Ovarian Neoplasms Vaginal Smears Training set business.industry Endometrial cancer Obstetrics and Gynecology Cancer Biomarker General Medicine Methylation DNA Methylation Middle Aged medicine.disease Endometrial Neoplasms 030104 developmental biology Oncology Case-Control Studies 030220 oncology & carcinogenesis DNA methylation Cancer research Feasibility Studies Original Article Female business Ovarian cancer |
| Zdroj: | Journal of Gynecologic Oncology |
| ISSN: | 2005-0399 2005-0380 |
| Popis: | Objective We hypothesized that DNA methylation of development-related genes may occur in endometrial cancer (EC)/ovarian cancer (OC) and may be detected in cervical scrapings. Methods We tested methylation status by quantitative methylation-specific polymerase chain reaction for 14 genes in DNA pools of endometrial and OC tissues. Tissues of EC/normal endometrium, OC/normal ovary, were verified in training set using cervical scrapings of 10 EC/10 OC patients and 10 controls, and further validated in the testing set using independent cervical scrapings in 30 EC/30 OC patients and 30 controls. We generated cutoff values of methylation index (M-index) from cervical scrapings to distinguish between cancer patients and control. Sensitivity/specificity of DNA methylation biomarkers in detecting EC and OC was calculated. Results Of 14 genes, 4 (PTGDR, HS3ST2, POU4F3, MAGI2) showed hypermethylation in EC and OC tissues, and were verified in training set. POU4F3 and MAGI2 exhibited hypermethylation in training set were validated in independent cases. The mean M-index of POU4F3 is 78.28 in EC and 20.36 in OC, which are higher than that in controls (6.59; p |
| Databáze: | OpenAIRE |
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