REM1.3's phospho-status defines its plasma membrane nanodomain organization and activity in restricting PVX cell-to-cell movement
| Autor: | Artemis Perraki, Julien Gronnier, Anthony Legrand, Véronique Germain, Anne-Flore Deroubaix, Sylvie German-Retana, Emmanuelle Bayer, Paul Gouguet, Marie Boudsocq, Sébastien Mongrand, Vincent Simon, Birgit Habenstein, Cyril Zipfel |
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| Přispěvatelé: | University of Zurich, Mongrand, Sébastien, Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Chimie et Biologie des Membranes et des Nanoobjets (CBMN), École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Universität Zürich [Zürich] = University of Zurich (UZH), European Project: 309858,EC:FP7:ERC,ERC-2012-StG_20111109,PHOSPHINNATE(2012), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB), Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Université Sciences et Technologies - Bordeaux 1-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Centre National de la Recherche Scientifique (CNRS), University of Zürich [Zürich] (UZH), Génomique, développement et pouvoir pathogène (GD2P), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Université Bordeaux Segalen - Bordeaux 2-Université Sciences et Technologies - Bordeaux 1-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR-16-CE20-0008,PotyMove,Facteurs cellulaires recrutés par les potyvirus pour leur transport intercellulaire : de nouvelles sources de résistance des plantes?(2016) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Virologie Arabidopsis 2405 Parasitology Plant Science Biochemistry 01 natural sciences Cell Movement Membrane proteins Arabidopsis thaliana In vitro kinase assay lcsh:QH301-705.5 Kinase Plasmodesmata Eukaryota résistance au virus Plants Potato virus X Cell biology Bioassays and Physiological Analysis Cellular Structures and Organelles virologie végétale réponse immunitaire Immunology Brassica Plasmodesma Microbiology 03 medical and health sciences 1311 Genetics Plant and Algal Models Genetics 1312 Molecular Biology Protein extraction 10211 Zurich-Basel Plant Science Center Protein kinase A Molecular Biology Plant Diseases Enzyme Assays virus phytopathogène 2403 Immunology Bacteria protéine remorin Cell Membrane arabidopsis thaliana fungi Organisms Biology and Life Sciences Proteins Phytopathologie et phytopharmacie [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy Potexvirus Cell membranes 030104 developmental biology chemistry Agrobacterium tumefaciens Animal Studies Capsid Proteins Parasitology Carrier Proteins lcsh:RC581-607 Protein Kinases 0301 basic medicine Leaves Agrobacteria [SDV]Life Sciences [q-bio] membrane plasmique 580 Plants (Botany) chemistry.chemical_compound Plant Microbiology 10126 Department of Plant and Microbial Biology Post-Translational Modification Phosphorylation Plant Proteins Extraction Techniques biology Plant Anatomy 2404 Microbiology [CHIM.MATE]Chemical Sciences/Material chemistry Plants Genetically Modified Experimental Organism Systems [SDV.IMM]Life Sciences [q-bio]/Immunology Signal transduction Research Article lcsh:Immunologic diseases. Allergy Phytopathology and phytopharmacy Research and Analysis Methods Model Organisms Virology [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Callose Cell Biology Phosphoproteins biology.organism_classification Plant Leaves Membrane protein lcsh:Biology (General) virus x de la pomme de terre 2406 Virology Biochemical Analysis 010606 plant biology & botany |
| Zdroj: | PLoS Pathogens, Vol 14, Iss 11, p e1007378 (2018) PLoS Pathogens PLoS Pathogens, Public Library of Science, 2018, 14 (11), pp.e1007378. ⟨10.1371/journal.ppat.1007378⟩ Plos Pathogens 11 (14), e1007378. (2018) PLoS Pathogens, 2018, 14 (11), pp.e1007378. ⟨10.1371/journal.ppat.1007378⟩ PLoS Pathogens, Public Library of Science, 2018, ⟨10.1371/journal.ppat.1007378⟩ PLoS Pathogens, Public Library of Science, 2018, 14 (11) |
| ISSN: | 1553-7366 1553-7374 |
| DOI: | 10.1371/journal.ppat.1007378⟩ |
| Popis: | Plants respond to pathogens through dynamic regulation of plasma membrane-bound signaling pathways. To date, how the plant plasma membrane is involved in responses to viruses is mostly unknown. Here, we show that plant cells sense the Potato virus X (PVX) COAT PROTEIN and TRIPLE GENE BLOCK 1 proteins and subsequently trigger the activation of a membrane-bound calcium-dependent kinase. We show that the Arabidopsis thaliana CALCIUM-DEPENDENT PROTEIN KINASE 3-interacts with group 1 REMORINs in vivo, phosphorylates the intrinsically disordered N-terminal domain of the Group 1 REMORIN REM1.3, and restricts PVX cell-to-cell movement. REM1.3's phospho-status defines its plasma membrane nanodomain organization and is crucial for REM1.3-dependent restriction of PVX cell-to-cell movement by regulation of callose deposition at plasmodesmata. This study unveils plasma membrane nanodomain-associated molecular events underlying the plant immune response to viruses. Author summary Viruses propagate in plants through membranous channels, called plasmodesmata, linking each cell to its neighboring cell. In this work, we challenge the role of the plasma membrane in the regulation of virus propagation. By studying the dynamics and the activation of a plant-specific protein called REMORIN, we found that the way this protein is organized inside the membrane is crucial to fulfill its function in the immunity of plants against viruses. |
| Databáze: | OpenAIRE |
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