Simultaneous determination of disulfiram and bupropion in human plasma of alcohol and nicotine abusers
| Autor: | Maria Augusta Raggi, Chiara Marcheselli, Francesco De Stefano, Maria Chiara Pieri, Lorenzo Somaini, Gilberto Gerra, Maria Addolorata Saracino |
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| Přispěvatelé: | M.A. Saracino, C. Marcheselli, L. Somaini, G. Gerra, F. De Stefano, M.C. Pieri, M.A. Raggi |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Detection limit
DIODE-ARRAY DETECTION Chromatography Molecular Structure PLASMA Calibration curve Chemistry Extraction (chemistry) Reversed-phase chromatography Tobacco Use Disorder Biochemistry High-performance liquid chromatography LIQUID CHROMATOGRAPHY Analytical Chemistry Standard curve Alcoholism BUPROPION Disulfiram medicine Humans Solid phase extraction Chromatography High Pressure Liquid DISULFIRAM medicine.drug |
| Popis: | An isocratic high-performance liquid-chromatographic method has been developed for the simultaneous determination of disulfiram and bupropion in human plasma samples. Analyses were carried out on a C(8) reversed-phase column using a mobile phase composed of 50% acetonitrile and 50% aqueous phosphate buffer, containing triethylamine. Diode-array detection was used, operating at a wavelength of 250 nm. For the clean-up of plasma samples, a solid phase extraction procedure, based on C(2) cartridges, was implemented. Extraction yields of the analytes were satisfactory, being always higher than 84%. The calibration curve was linear over the 5-500 ng mL(-1) plasma concentration range for both disulfiram and bupropion. The method showed a high sensitivity (limit of detection of 1.5 ng mL(-1)) and satisfactory precision, selectivity and accuracy. The application to human plasma samples obtained from some alcohol and nicotine abusers also gave good results. |
| Databáze: | OpenAIRE |
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