Mutagenicity and cytotoxicity of haloethanes as studied in the CHO/HGPRT system
| Autor: | Eng-Lay Tan, Abraham W. Hsie |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
endocrine system
Hypoxanthine Phosphoribosyltransferase Ethyl methanesulfonate Guanine Ethylene Dibromide Ethylene Dichloride Toxicology Cell Line chemistry.chemical_compound Cricetulus Cricetinae Genetics Hydrocarbons Chlorinated Animals Ethylene Dichlorides Cytotoxicity Hypoxanthine Hydrocarbons Halogenated Mutagenicity Tests Chinese hamster ovary cell Ovary food and beverages Hydrocarbons Brominated chemistry Biochemistry Hypoxanthine-guanine phosphoribosyltransferase Female Mutagens |
| Zdroj: | Mutation research. 90(2) |
| ISSN: | 0027-5107 |
| Popis: | When haloethanes were being tested as direct-acting agents in the Chinese hamster ovary cell/hypoxanthine—guanine phosphoribosyl transferase (CHO/HGPRT) system, ethylene dibromide (EtBr 2 ) exhibited more cytotoxic and mutagenic activity than ethylene dichloride (EtCl 2 ), and the mixed halogenated congener ethylene bromochloride (EtBrCl) had an intermediate effect. On a molar basis, the relative mutagenic activity of EtBr 2 : EtBrCl : EtCl 2 was approximately 100 : 6 : 1. Cell survival was reduced to 50% by approx. 3, 6 and 50 mM of EtBr 2 , EtBrCl and EtCl 2 , respectively, and declined precipitously with increasing concentrations of the haloethanes. When these 3 haloethanes were assayed in the presence of S9, there was a 5–25-fold increase in cytotoxicity; however, only EtBrCl and EtCl 2 also showed a concomitant increase in mutagenicity of 4-fold. The mutagenicity of EtBr 2 remained unchanged when assayed in the presence of S9. When NADP was omitted from the S9 mix, which contains a NADPH-regenerating system, the increase in cytotoxicity and mutagenicity observed with the complete S9 mix was abolished. EtBr 2 was shown to possess a molar equivalent mutagenic activity to ethyl methanesulfonate under the conditions of the assay. The cytotoxicity of EtBr 2 increased as the time of treatment increased up to 24 h, while mutation induction appeared to peak at around 5 h. |
| Databáze: | OpenAIRE |
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