Coordinate control of rat liver lipogenic enzymes by insulin
| Autor: | John W. Porter, M.R. Lakshmanan, Don A. Kleinsek, Gene C. Ness, Carl M. Nepokroeff, Richard A. Muesing |
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| Rok vydání: | 1974 |
| Předmět: |
Male
L-Serine Dehydratase medicine.medical_specialty Time Factors medicine.medical_treatment Biophysics Malic enzyme Glucosephosphate Dehydrogenase Biology Biochemistry Glucagon Streptozocin Cytosol Malate Dehydrogenase Internal medicine Glucokinase Diabetes Mellitus medicine Animals Insulin Carbon Radioisotopes Molecular Biology chemistry.chemical_classification Phosphogluconate Dehydrogenase Fatty acid Lipids Isocitrate Dehydrogenase Pyruvate carboxylase Kinetics Isocitrate dehydrogenase Enzyme Endocrinology Liver chemistry Starvation ATP Citrate (pro-S)-Lyase Fatty Acid Synthases Acetyl-CoA Carboxylase |
| Zdroj: | Archives of Biochemistry and Biophysics. 162:340-344 |
| ISSN: | 0003-9861 |
| Popis: | Recent evidence has established that insulin is required for the dietary induction of rat liver fatty acid synthetase [ Proc. Nat. Acad. Sci. USA 69 , 3516 (1972)]. Since other hepatic lipogenic enzymes as well as fatty acid synthetase exhibit coordinate adaptation to nutritional changes [ Advan. Enzyme Regul. 10 , 187(1972)], the role of insulin in the dietary induction of these enzymes has been investigated. When a high-carbohydrate, fat-free diet was fed to diabetic rats previously fasted for 48 hr, insulin was shown to be required for the dietary induction of acetyl-CoA carboxylase, citrate cleavage enzyme, malic enzyme, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, fatty acid synthetase, and glucokinase. Activity of serine dehydrase, selected as a model gluconeogenic enzyme, was increased in diabetic rats, whereas insulin treatment reduced the activity of this enzyme during the course of refeeding. The behavior of serine dehydrase was consistent with its gluconeogenic role. The activity of the cytosol isocitrate dehydrogenase did not change during refeeding in the diabetic or insulin-treated diabetic rat. Glucagon, the physiological antagonist of insulin, inhibited the increase in activity of each of the lipogenic enzymes requiring insulin for induction. Our results indicate that insulin is required for the coordinate regulation of the lipogenic enzymes of mammalian liver. |
| Databáze: | OpenAIRE |
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