COMPARISON OF SELECTION RESPONSES ON BODY WEIGHT UNDER DIVERGENT LARVAL DENSITY CONDITIONS IN DROSOPHILA PSEUDOOBSCURA
| Autor: | Ken-Ichi Kojima, Richard R. Frahm |
|---|---|
| Rok vydání: | 1966 |
| Předmět: |
Male
business.product_category Population Investigations Environment Body weight Drosophila pseudoobscura Animal science Genetics Bottle Animals Bottle cap education Drosophila education.field_of_study Larva biology Ecology Body Weight fungi biology.organism_classification Genetics Population Female Cage business |
| Zdroj: | Genetics. 54:625-637 |
| ISSN: | 1943-2631 |
| DOI: | 10.1093/genetics/54.2.625 |
| Popis: | Description of bose populatiom The basic genetic material used in this study was a randommating cage population of Drosophila pseudoobscura formed by intermating flies from two parental populations, Mono and Mather. The history of the Mono and Mather cage populations was given by KOJIMA and KELLEHER (1963). The sensitivity of adult body weight to the level of larval density in the culture bottles is indicated by the following: for egg densities of 50, 100, 200 and 4Nl eggs per bottle, the average body weights were 1.53, 1.25, 1.20 and 1.00 mg, respectively. Eclosion percentage was found to vary inversely with larval density. The percentages of adult flies emerging from the two densities considered in this study (50 and 200 eggs per bottle) were approximately 90 and 65, respectively. Culture methods: All experimental flies were kept at a controlled temperature of 19°C and high relative humidity. The flies were reared on a cream of wheat medium poured to a depth of approximately one inch in half-pint milk bottles. A drop of dried baker’s yeast water suspension was added just prior to placing the eggs in the bottle. The bottles were plugged with standard milk bottle caps. Egg collection and weighing procedure: After the desired matings were made, the impregnated females were placed in egg-laying bottles (empty half-pint milk bottles). The females laid their eggs on an agar-molasses-charcoal medium that had been spread on the underside of the caps used to plug the egg-laying bottles. The desired number of eggs (50 or 200) was counted under a dissecting microscope and transferred to the culture bottles. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|