Different Expression of Hypoxic and Angiogenic Factors in Human Endometriotic Lesions

Autor: Charles Chapron, Stefano Luisi, Felice Petraglia, Antonella Naldini, Irene Filippi, Patrizia Carrarelli, Frédéric Batteux
Rok vydání: 2016
Předmět:
Adult
Vascular Endothelial Growth Factor A
0301 basic medicine
Pathology
medicine.medical_specialty
Angiogenesis
Endometriosis
hypoxia-inducible factors
Endometrium
Young Adult
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Gene expression
ovarian endometrioma
Basic Helix-Loop-Helix Transcription Factors
Humans
Medicine
Receptor
030219 obstetrics & reproductive medicine
vascular endothelial growth factor
Neovascularization
Pathologic
business.industry
protease-activated receptors
Obstetrics and Gynecology
deep infiltrating endometriosis
Hypoxia-Inducible Factor 1
alpha Subunit
medicine.disease
Vascular endothelial growth factor
deep infiltrating endometriosis
hypoxia-inducible factors
ovarian endometrioma
protease-activated receptors
vascular endothelial growth factor
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
Hypoxia-inducible factors
chemistry
Cancer research
Female
Receptors
Thrombin
Signal transduction
business
Zdroj: Reproductive Sciences. 23:492-497
ISSN: 1933-7205
1933-7191
Popis: Endometriosis is associated with local angiogenic and hypoxic mechanisms. Indeed, peritoneal fluid of women with endometriosis generates a specific microenvironment to support the growth and development of ectopic endometrial tissues. The association between proangiogenic markers and hypoxic processes in different endometriosis phenotypes was investigated in the present study, analyzing the expression of several genes, related to hypoxic signaling pathway and involved in angiogenic processes, in nonpregnant women with different forms of endometriosis. Samples of ovarian endometrioma (OMA; n = 16) or deep infiltrating endometriosis (DIE; n = 11) were collected, and in addition, control endometrium was collected from healthy women by hysteroscopy. The gene expression of the hypoxia-inducible factors (HIF) 1/2α, protease-activated receptors (PARs) ¼, and vascular endothelial growth factor (VEGF) A was evaluated by quantitative reverse-transcription polymerase chain reaction. Ovarian endometrioma expresses high levels of HIF-1/2α, PAR-1/4, and VEGF-A, while DIE did not show significantly different gene expression compared to endometrium from unaffected women. A positive correlation between the expression of HIF-1/2α and VEGF-A mRNA was observed in OMA. The overall data point out that the heterogeneity of the disease reflects differences in expression levels of genes associated with hypoxia and angiogenesis, suggesting that such conditions may have an active role in the development of the disease.
Databáze: OpenAIRE
Externí odkaz: