Circulating miRNAs in acute new-onset atrial fibrillation and their target mRNA network

Autor:	Jéssica Nayara Góes de Araújo, Adriana Augusto de Rezende, André Ducati Luchessi, Ananília Medeiros Gomes da Silva, Ana Eloísa Melo Novaes, Vivian Nogueira Silbiger, Mariana Borges Lopes, Antônio Amorim de Araújo Filho, Júlio César Vieira de Sousa, Katiene Macêdo de Oliveira, Mario Hiroyuki Hirata
Rok vydání:	2018
Předmět:	0301 basic medicine Circulating mirnas Oncology Adult Male medicine.medical_specialty Pilot Projects 030204 cardiovascular system & hematology Pathogenesis 03 medical and health sciences 0302 clinical medicine Fibrosis Physiology (medical) Internal medicine microRNA Atrial Fibrillation medicine Humans Gene Regulatory Networks Circulating MicroRNA RNA Messenger Aged Fibrillation business.industry Atrial fibrillation Middle Aged medicine.disease 030104 developmental biology Apoptosis Acute Disease Target mrna Female medicine.symptom Cardiology and Cardiovascular Medicine business
Zdroj:	Journal of cardiovascular electrophysiology. 29(8)
ISSN:	1540-8167
Popis:	Background MicroRNAs (miRNAs) are involved in the pathogenesis of atrial fibrillation (AF), acting on development and progression. Our pilot study investigated the expression of six miRNAs and their miRNA-mRNA interactions in patients with acute new-onset AF, well-controlled AF, and normal sinus rhythm (controls). Methods and results Plasma of acute new-onset AF patients (n = 5) was collected in the emergency room when patients presented with irregular and fast-atrial fibrillation rhythm. Samples from well-controlled AF (n = 16) and control (n = 15) patients were collected during medical appointments following an ECG. Expression of miR-21, miR-133a, miR-133b, miR-150, miR-328, and miR-499 was analyzed by real-time PCR. Ingenuity Pathway Analysis and the TargetScan database identified the top 30 mRNA targets of these miRNA, seeking the miRNA-mRNA interactions in cardiovascular process. Increased expression of miR-133b (1.4-fold), miR-328 (2.0-fold), and miR-499 (2.3-fold) was observed in patients with acute new-onset AF, compared with well-controlled AF and control patients. Decreased expression of miR-21 was seen in patients with well-controlled AF compared to those with acute new-onset AF and controls (0.6-fold). The miRNA-mRNA interaction demonstrated that SMAD7 and FASLG genes were the targets of miR-21, miR-133b, and miR-499 and were directly related to AF, being involved in apoptosis and fibrosis. Conclusion The miRNAs had different expression profiles dependent on the AF condition, with higher expression in the acute new-onset AF than well-controlled AF. Clinically, this may contribute to an effective assessment for patients, leading to early detection of AF and monitoring to reduce the risk of other serious cardiovascular events.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9214435ae746306ac08a89159625ff14 https://pubmed.ncbi.nlm.nih.gov/29676832 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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