Noelin1 Affects Lateral Mobility of Synaptic AMPA Receptors

Autor: Norbert Babai, Volker Mack, Huibert D. Mansvelder, Miguel A. Gonzalez-Lozano, Renato Frischknecht, Johannes C. Lodder, August B. Smit, Ka Wan Li, Nikhil J. Pandya, Christian Seeger, Yvonne Gouwenberg, Martin Heine, U. Helena Danielson, Sabine Spijker
Přispěvatelé: Molecular and Cellular Neurobiology, Integrative Neurophysiology, Amsterdam Neuroscience - Cellular & Molecular Mechanisms, AIMMS, Amsterdam Neuroscience - Neurodegeneration, Amsterdam Neuroscience - Mood, Anxiety, Psychosis, Stress & Sleep, Amsterdam Neuroscience - Compulsivity, Impulsivity & Attention, Center for Neurogenomics and Cognitive Research
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Molecular biology
receptor mobility
Hippocampus
Extracellular matrix
Mice
0302 clinical medicine
Desensitization (telecommunications)
Biologiska vetenskaper
AMPA receptor
Receptor
Cells
Cultured
chemistry.chemical_classification
Extracellular Matrix Proteins
Neuronal Plasticity
musculoskeletal
neural
and ocular physiology
Glutamate receptor
Long-term potentiation
Biological Sciences
Farmakologi och toxikologi
Synapse
Protein Transport
Protein Binding
glutamate receptor
Biophysics
AMPAR-associated protein
Pharmacology and Toxicology
Article
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
synapse function
Animals
Humans
Receptors
AMPA
Biology
Glycoproteins
synaptic plasticity
Mice
Inbred C57BL
HEK293 Cells
030104 developmental biology
chemistry
nervous system
Synapses
Synaptic plasticity
Glycoprotein
030217 neurology & neurosurgery
Zdroj: Cell Reports
Pandya, N J, Seeger, C, Babai, N, Gonzalez-Lozano, M A, Mack, V, Lodder, J C, Gouwenberg, Y, Mansvelder, H D, Danielson, U H, Li, K W, Heine, M, Spijker, S, Frischknecht, R & Smit, A B 2018, ' Noelin1 Affects Lateral Mobility of Synaptic AMPA Receptors ', Cell Reports, vol. 24, no. 5, pp. 1218-1230 . https://doi.org/10.1016/j.celrep.2018.06.102
Cell Reports, 24(5), 1218-1230. Cell Press
PubMed Central
Europe PubMed Central
Datacite
DANS (Data Archiving and Networked Services)
NARCIS
UnpayWall
ORCID
Microsoft Academic Graph
Publikationer från Uppsala Universitet
ISSN: 2211-1247
DOI: 10.1016/j.celrep.2018.06.102
Popis: Pandya et al. find that the secreted protein Noelin1 binds AMPA-type glutamate receptors with high affinity. They show a role in limiting receptor mobility and keeping receptors at synaptic sites in a subunit-specific way. This is the first evidence for a secreted auxiliary subunit in mobility and plasticity of glutamate receptors.
Graphical Abstract
Highlights • Noelin1 interacts with high affinity to AMPA receptors (AMPARs) • Noelin1 is secreted upon cellular stimulation • (Extra)synaptic AMPAR mobility, but not channel properties, are affected by Noelin1 • Reducing synaptic AMPAR lateral mobility by Noelin1 limits synaptic plasticity
Summary Lateral diffusion on the neuronal plasma membrane of the AMPA-type glutamate receptor (AMPAR) serves an important role in synaptic plasticity. We investigated the role of the secreted glycoprotein Noelin1 (Olfactomedin-1 or Pancortin) in AMPAR lateral mobility and its dependence on the extracellular matrix (ECM). We found that Noelin1 interacts with the AMPAR with high affinity, however, without affecting rise- and decay time and desensitization properties. Noelin1 co-localizes with synaptic and extra-synaptic AMPARs and is expressed at synapses in an activity-dependent manner. Single-particle tracking shows that Noelin1 reduces lateral mobility of both synaptic and extra-synaptic GluA1-containing receptors and affects short-term plasticity. While the ECM does not constrain the synaptic pool of AMPARs and acts only extrasynaptically, Noelin1 contributes to synaptic potentiation by limiting AMPAR mobility at synaptic sites. This is the first evidence for the role of a secreted AMPAR-interacting protein on mobility of GluA1-containing receptors and synaptic plasticity.
Databáze: OpenAIRE
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