Translation of type IV procollagen messenger RNA from cultured cat retinal pigment epithelial cells
| Autor: | Weiye Li, Lawrence E. Stramm, Gustavo D. Aguirre, Shuyu Shen, John H. Rockey, George A. Robertson |
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| Rok vydání: | 1985 |
| Předmět: |
Biology
Cellular and Molecular Neuroscience chemistry.chemical_compound medicine Animals RNA Messenger Pigment Epithelium of Eye Cells Cultured Electrophoresis Agar Gel Retina Messenger RNA Immune Sera RNA Translation (biology) Retinal General Medicine Molecular biology Sensory Systems Ophthalmology Procollagen peptidase medicine.anatomical_structure chemistry Cell culture Cytoplasm Protein Biosynthesis Cats Collagen Procollagen |
| Zdroj: | Ophthalmic research. 17(4) |
| ISSN: | 0030-3747 |
| Popis: | The activity of type IV procollagen mRNA was detected in the total cytoplasmic RNA prepared from normal feline retinal pigment epithelial cells in culture. The translation products contained two distinct bands in the pro alpha chain region on SDS-PAGE, which were sensitive to collagenase digestion. Corresponding bands were identified in the immunoprecipitate of the translation products after reaction with an anti-type IV collagen antiserum and separation by protein A-Sepharose column chromatography. |
| Databáze: | OpenAIRE |
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