Development of a Miniaturized Glucose Monitoring System by Combining a Needle-Type Glucose Sensor With Microdialysis Sampling Method: Long-term subcutaneous tissue glucose monitoring in ambulatory diabetic patients
| Autor: | Kenro Nishida, Michiharu Sakakida, Y. Hashiguchi, Motoaki Shichiri, T. Uemura, K. Kajiwara |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Blood Glucose medicine.medical_specialty Microdialysis Endocrinology Diabetes and Metabolism Biosensing Techniques Reference Values In vivo Internal medicine Diabetes mellitus Diabetes Mellitus Internal Medicine medicine Humans Monitoring Physiologic Glycated Hemoglobin Advanced and Specialized Nursing Miniaturization business.industry Glucose Measurement Needle type Monitoring system Equipment Design medicine.disease Diabetes Mellitus Type 1 Endocrinology medicine.anatomical_structure Diabetes Mellitus Type 2 Ambulatory Regression Analysis business Subcutaneous tissue Biomedical engineering |
| Zdroj: | Diabetes Care. 17:387-396 |
| ISSN: | 1935-5548 0149-5992 |
| DOI: | 10.2337/diacare.17.5.387 |
| Popis: | OBJECTIVE To develop a reliable and practical glucose monitoring system by combining a needle-type glucose sensor with a microdialysis sampling technique for long-term subcutaneous tissue glucose measurements. RESEARCH DESIGN AND METHODS A microdialysis Cuprophan hollowfiber probe (inner diameter, 0.20 mm; length, 15 mm) was perfused with isotonic saline solution (120 μl/h) and glucose concentrations in the dialysate were measured by a needle-type glucose sensor extracorporeally. This system was tested both in vitro and in vivo. Subcutaneous tissue glucose concentrations were then monitored continuously in 5 healthy and 8 diabetic volunteers for 7 to 8 days. A hollow-fiber probe was inserted into the abdominal subcutaneous tissue. RESULTS This monitoring system achieved excellent results in vitro. Subcutaneous tissue glucose concentrations were measured in a wide range from 1.7 to >027.8 mM glucose, with a time delay of 6.9 ±1.2 min associated with a rise in glucose and 8.8 ±1.6 min with a fall in the glucose level (means ± SE). The overall correlation between subcutaneous tissue (Y) and blood (X) glucose concentration was Y = 1.08X ± 0.19 (r = 0.99). The subcutaneous tissue glucose concentration could be monitored precisely for 4 days without any in vivo calibrations and for 7 days by introducing in vivo calibrations. CONCLUSIONS Glycemic excursions could be monitored precisely in the subcutaneous tissue by this microdialysis sampling method with a needle-type glucose sensor in ambulatory diabetic patients. |
| Databáze: | OpenAIRE |
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