Gene Regulation via the Combination of Transcription Factors in the INDETERMINATE DOMAIN and GRAS Families
| Autor: | Shun Ikeya, Akiko Kozaki, Atsushi Kobayashi, Takuya Aoyanagi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Subfamily SCARECROW (SCR) lcsh:QH426-470 Arabidopsis Repressor 01 natural sciences behavioral disciplines and activities Article Mixed Function Oxygenases INDETERMINATE DOMAIN (IDD) family SHORT-ROOT (SHR) 03 medical and health sciences chemistry.chemical_compound Genetics transcription factor (TF) Promoter Regions Genetic SCARECROW-LIKE 3 (SCL3) REPRESSOR of ga1-3 (RGA) Gene Transcription factor Genetics (clinical) Regulation of gene expression biology Arabidopsis Proteins Chemistry GA3 oxidase (GA3ox) 1 Promoter biology.organism_classification Gibberellins Cell biology DNA-Binding Proteins Repressor Proteins lcsh:Genetics 030104 developmental biology Multigene Family GRAS family DELLA Co-Repressor Proteins DNA Transcription Factors 010606 plant biology & botany |
| Zdroj: | Genes, Vol 11, Iss 613, p 613 (2020) Genes Volume 11 Issue 6 |
| ISSN: | 2073-4425 |
| Popis: | INDETERMINATE DOMAIN (IDD) family proteins are plant-specific transcription factors. Some Arabidopsis IDD (AtIDD) proteins regulate the expression of SCARECROW (SCR) by interacting with GRAS family transcription factors SHORT-ROOT (SHR) and SCR, which are involved in root tissue formation. Some AtIDD proteins regulate genes involved in the synthesis (GA3ox1) or signaling (SCL3) of gibberellic acid (GA) by interacting with DELLA proteins, a subfamily of the GRAS family. We analyzed the DNA binding properties and protein&ndash protein interactions of select AtIDD proteins. We also investigated the transcriptional activity of the combination of AtIDD and GRAS proteins (AtIDD proteins combined with SHR and SCR or with REPRESSOR of ga1-3 (RGA)) on the promoters of SCR, SCL3, and GA3ox1 by conducting a transient assay using Arabidopsis culture cells. Our results showed that the SCR promoter could be activated by the IDD and RGA complexes and that the SCL3 and GA3ox1 promoters could be activated by the IDD, SHR, and SCR complexes, indicating the possibility that these complexes regulate and consequently coordinate the expression of genes involved in GA synthesis (GA3ox1), GA signaling (SCL3), and root formation (SCR). |
| Databáze: | OpenAIRE |
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