Cell density increases Bcl-2 and Bcl-x expression in addition to survival of cultured cerebellar granule neurons
| Autor: | Hans Thoenen, Theologos M. Michaelidis, Susanne Hamnér, Dan Lindholm, Jonathan D. Cooper, Y. Ohga, Ute Zirrgiebel |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Programmed cell death
Cerebellum Cell Survival Blotting Western bcl-X Protein Gene Expression Cell Count Biology Neurotrophic factors Proto-Oncogene Proteins Gene expression medicine Animals RNA Messenger Insulin-Like Growth Factor I Cells Cultured bcl-2-Associated X Protein Neurons Regulation of gene expression Messenger RNA Brain-Derived Neurotrophic Factor General Neuroscience Genes bcl-2 Rats Cell biology Blot medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 nervous system Signal transduction Neuroscience |
| Zdroj: | Neuroscience. 73:913-917 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/0306-4522(96)00204-7 |
| Popis: | The proto-oncogene bcl-2 and its family members, bcl-x and bax are recognized as major regulators of cell death and survival. Although Bcl-2 and Bcl-x are expressed in brain, little is known how they are regulated in neurons. Here we have studied the expression of bcl-2, bcl-xL and bax mRNA in rat cerebellar granule neurons cultured under conditions which influence neuron survival. Insulin-like growth factor-1 and brain-derived neurotrophic factor supported the survival of these neurons, but affected neither the expression of bcl-2, bcl-xL nor bax mRNA. In contrast, bcl-2 and bcl-xL mRNAs were up-regulated in cerebellar granule neurons plated at high density exhibiting an increased neuronal survival. Western blots showed that cell density also increased Bcl-2 protein level. However, conditioned medium from dense cultures did not affect the level of bcl-2 mRNA nor survival of the neurons. This suggests that cell density promotes survival and regulates Bcl-2 expression in cerebellar granule neurons through a signaling pathway different from known neurotrophic factors. |
| Databáze: | OpenAIRE |
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