Lens differentiation is characterized by stage-specific changes in chromatin accessibility correlating with differentiation state-specific gene expression
| Autor: | Marc Kantorow, A. Sue Menko, Daniel Chauss, Joshua Disatham, Rifah Gheyas, Lisa A. Brennan, Lauren Daley, David Blanco |
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| Rok vydání: | 2019 |
| Předmět: |
BFSP2
Biology 03 medical and health sciences 0302 clinical medicine Consensus Sequence Lens Crystalline Gene expression Animals RNA Messenger Molecular Biology Gene Transcription factor 030304 developmental biology Regulation of gene expression 0303 health sciences Binding Sites Genome Base Sequence RBPJ Gene Expression Regulation Developmental Cell Differentiation DNA Cell Biology Chromatin Cell biology Regulatory sequence Chickens Biomarkers 030217 neurology & neurosurgery Protein Binding Transcription Factors Developmental Biology |
| Zdroj: | Developmental Biology. 453:86-104 |
| ISSN: | 0012-1606 |
| Popis: | Changes in chromatin accessibility regulate the expression of multiple genes by controlling transcription factor access to key gene regulatory sequences. Here, we sought to establish a potential function for altered chromatin accessibility in control of key gene expression events during lens cell differentiation by establishing genome-wide chromatin accessibility maps specific for four distinct stages of lens cell differentiation and correlating specific changes in chromatin accessibility with genome-wide changes in gene expression. ATAC sequencing was employed to generate chromatin accessibility profiles that were correlated with the expression profiles of over 10,000 lens genes obtained by high-throughput RNA sequencing at the same stages of lens cell differentiation. Approximately 90,000 regions of the lens genome exhibited distinct changes in chromatin accessibility at one or more stages of lens differentiation. Over 1000 genes exhibited high Pearson correlation coefficients (r > 0.7) between altered expression levels at specific stages of lens cell differentiation and changes in chromatin accessibility in potential promoter (−7.5kbp/+2.5kbp of the transcriptional start site) and/or other potential cis-regulatory regions ( ±10 kb of the gene body). Analysis of these regions identified consensus binding sequences for multiple transcription factors including members of the TEAD, FOX, and NFAT families of transcription factors as well as HIF1a, RBPJ and IRF1. Functional mapping of genes with high correlations between altered chromatin accessibility and differentiation state-specific gene expression changes identified multiple families of proteins whose expression could be regulated through changes in chromatin accessibility including those governing lens structure (BFSP1,BFSP2), gene expression (Pax-6, Sox 2), translation (TDRD7), cell-cell communication (GJA1), autophagy (FYCO1), signal transduction (SMAD3, EPHA2), and lens transparency (CRYBB1, CRYBA4). These data provide a novel relationship between altered chromatin accessibility and lens differentiation and they identify a wide-variety of lens genes and functions that could be regulated through altered chromatin accessibility. The data also point to a large number of potential DNA regulatory sequences and transcription factors whose functional analysis is likely to provide insight into novel regulatory mechanisms governing the lens differentiation program. |
| Databáze: | OpenAIRE |
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