An all-in-one point-of-care testing device for multiplexed detection of respiratory infections

Autor: Teixeira, William, Pallás-Tamarit, Yeray, Juste-Dolz, Augusto Miguel, Sena-Torralba, Amadeo, Gozalbo-Rovira, Roberto, Rodríguez-Díaz, Jesús, Navarro, David, Carrascosa Rubio, Javier, Giménez-Romero, David, Maquieira Catala, Angel, Morais, Sergi
Rok vydání: 2022
Předmět:
Zdroj: BIOSENSORS & BIOELECTRONICS
r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe
instname
ISSN: 0956-5663
DOI: 10.1016/j.bios.2022.114454
Popis: [EN] The impact of the COVID-19 pandemic has reinforced the need for rapid, cost-effective, and reliable point-of-care testing (POCT) devices for massive population screening. The co-circulation of SARS-CoV-2 with several seasonal respiratory viruses highlights the need for multiplexed biosensing approaches. Herein, we present a fast and robust all-in-one POCT device for parallel viral antigen and serological analysis. The biosensing approach consists of a functionalized polycarbonate disc-shaped surface with microfluidic structures, where specific bioreagents are immobilized in microarray format, and a portable optoelectronic analyzer. The biosensor quantifies the concentration of viral antigens and specific immunoglobulins G and M for SARS-CoV-2, influenza A/B, adenovirus, and respiratory syncytial virus, using 30¿¿L of a sample. The semi-automated analysis of 6 samples is performed in 30¿min. Validation studies performed with 135 serum samples and 147 nasopharyngeal specimens reveal high diagnostic sensitivity (98¿100%) and specificity (84¿98%), achieving an excellent agreement (¿¿=¿0.937) with commercial immunoassays, which complies with the World Health Organization criteria for POC COVID-19 diagnostic tests. The versatility of the POCT device paves the way for the detection of other pathogens and analytes in the incoming post-pandemic world, integrating specific bioreagents against different variants of concerns and interests.
W. T and A. J-D acknowledge the financial support for the Ph.D. studies (PAID-19-01-06 and FPI-UPV 2017 grants, respectively). S.M awards grant PID2019-110713RB-I00 funded by MCIN/AEI/10.13039/501100011033 and by "ERDF A way of making Europe." A.M awards grant DOGV no. 8815, May 19, 2020, pp. 16712-16726. The research was also supported by the Generalitat Valenciana (PROMETEO/2020/094, Proyecto "88/2021"). The authors gratefully acknowledge the outstanding contribution of Alicia Parra, Violeta Espada, Roberto Renuncio, and Jose A. Marzal for collecting the nasopharyngeal swab samples from the Health Centre of the Universitat Politecnica de Valencia. We also gratefully thank Dr. L. Mira and G. Rios for carefully reading this manuscript and the four anonymous reviewers for their insightful comments and suggestions.
Databáze: OpenAIRE
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