Effect of the flavonoid baicalin on the proliferative capacity of bovine mammary cells and their ability to regulate oxidative stress
| Autor: | Emilien Dupuis, Florence Gondret, Marie-Hélène Perruchot, Fabrice Robert, Frederic Dessauge, H. Quesnel |
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| Přispěvatelé: | Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Conseils et Compétences en Productions Animales (CCPA), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Veterinary Medicine
Antioxidant medicine.medical_treatment lcsh:Medicine Mammary epithelial cell Pharmacology medicine.disease_cause General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Biologie animale medicine Dairy cow cellule épithéliale mammaire dairy cows Viability assay Baicalin Agricultural Science 030304 developmental biology Animal biology chemistry.chemical_classification cellular proliferation prolifération cellulaire 0303 health sciences Reactive oxygen species apoptose biology General Neuroscience viabilité cellulaire [SDV.BA]Life Sciences [q-bio]/Animal biology lcsh:R General Medicine stress oxydant biology.organism_classification chemistry Apoptosis Catalase Oxidative stress vache laitière biology.protein Scutellaria baicalensis Cell culture General Agricultural and Biological Sciences 030217 neurology & neurosurgery |
| Zdroj: | PeerJ PeerJ, PeerJ, 2019, 7, ⟨10.7717/peerj.6565⟩ PeerJ, PeerJ, 2019, ⟨10.7717/peerj.6565⟩ PeerJ, Vol 7, p e6565 (2019) PeerJ (7), . (2019) |
| ISSN: | 2167-8359 |
| Popis: | Background High-yielding dairy cows are prone to oxidative stress due to the high metabolic needs of homeostasis and milk production. Oxidative stress and inflammation are tightly linked; therefore, anti-inflammatory and/or natural antioxidant compounds may help improve mammary cell health. Baicalin, one of the major flavonoids in Scutellaria baicalensis, has natural antioxidant and anti-inflammatory properties in various cell types, but its effects on bovine mammary epithelial cells (BMECs) have not been investigated. Methods Explants from bovine mammary glands were collected by biopsy at the peak of lactation (approximately 60 days after the start of lactation) (n = three animals) to isolate BMECs corresponding to mature secretory cells. Cell viability, apoptosis, proliferative capacity and reactive oxygen species (ROS) production by BMECs were measured after increasing doses of baicalin were added to the culture media in the absence or presence of H2O2, which was used as an in vitro model of oxidative stress. Results Low doses of baicalin (1–10 µg/mL) had no or only slightly positive effects on the proliferation and viability of BMECs, whereas higher doses (100 or 200 µg/mL) markedly decreased BMEC proliferation. Baicalin decreased apoptosis rate at low concentrations (10 µg/mL) but increased apoptosis at higher doses. ROS production was decreased in BMECs treated with increasing doses of baicalin compared with untreated cells, and this decreased production was associated with increased intracellular concentrations of catalase and NRF-2. Irrespective of the dose, baicalin pretreatment attenuated H2O2-induced ROS production. Discussion These results indicate that baicalin exerts protective antioxidant effects on bovine mammary cells. This finding suggests that baicalin could be used to prevent oxidative metabolic disorders in dairy cows. |
| Databáze: | OpenAIRE |
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