Circadian variations of bone marrow engraftability
| Autor: | Christina I. McAuliffe, Lionel D'Hondt, Chung-Cheng Hsieh, Gerald A. Colvin, Kimberly Stencel, Jane E. Carlson, Houri Habibian, Jeffrey Damon, Judith Reilly, Jean-Francois Lambert, Peter J. Quesenberry, Mark S. Dooner |
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| Přispěvatelé: | UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Service d'oncologie médicale |
| Rok vydání: | 2004 |
| Předmět: |
Male
Bone marrow transplant medicine.medical_specialty Time Factors Physiology Transplantation Heterologous Clinical Biochemistry Congenic Bone Marrow Cells Cell Count Mice Inbred Strains Spleen Thymus Gland Biology Tritium Colony-Forming Units Assay Mice Internal medicine medicine Animals Cell Lineage Femur Circadian rhythm Progenitor cell Cells Cultured Bone Marrow Transplantation Tibia Genetic Variation Cell Biology Circadian Rhythm Mice Inbred C57BL Haematopoiesis medicine.anatomical_structure Endocrinology Immunology Seasons Halo Bone marrow Thymidine |
| Zdroj: | Journal of cellular physiology, Vol. 200, no. 1, p. 63-70 (2004) |
| ISSN: | 1097-4652 0021-9541 |
| DOI: | 10.1002/jcp.20032 |
| Popis: | Circadian rhythms exist for hematopoiesis, but little is known about circadian variation of bone marrow engraftability and host “acceptability”. Using a B6.SJL to C57BL/6J congenic transplant model, we chose 3-times with light on: Hours After Light Onset (HALO) 4, 8, and 12 and 3-times with light off: HALO 16, 20, and 24. The mice were conditioned on a 12-h light/dark cycles. Recipient mice (100 cGy) received 40 million cells. We demonstrated a significant variation of bone marrow engraftability into bone marrow, spleen, and thymus when donor animals were subjected to changes in their light/dark cycles. Two statistically significant nadirs in all three organs were observed at HALO 8 and 24 in experiments carried out in July, while an identical set of experiments in February analyzing engraftment in marrow and spleen showed nadirs at HALO 8, but not at HALO 24. Marrow progenitors from the July experiments showed nadirs at HALO 12 and 24. The percentage of progenitors in S phase peaked at HALO 8 and 24. Interestingly, there were no changes in the ability of host to accept grafts with changes in the light/dark cycles of host animals. Circadian variations of bone marrow engraftability are important and should be considered in bone marrow transplant strategies. © 2004 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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