Immunogenicity and Protection Against Influenza H7N3 in Mice by Modified Vaccinia Virus Ankara Vectors Expressing Influenza Virus Hemagglutinin or Neuraminidase
| Autor: | Wei Wang, Jerry P. Weir, Carol D. Weiss, Jin Gao, Jackeline Soto, Maryna C. Eichelberger, Vajini Atukorale, Clement A. Meseda |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
China viruses Cross Protection lcsh:Medicine Hemagglutinin (influenza) Neuraminidase Hemagglutinin Glycoproteins Influenza Virus Vaccinia virus medicine.disease_cause Antibodies Viral Influenza A Virus H7N9 Subtype complex mixtures Virus Article 03 medical and health sciences chemistry.chemical_compound Influenza A Virus H7N3 Subtype Mice Viral Proteins Immunogenicity Vaccine Orthomyxoviridae Infections Influenza Human Influenza A virus medicine Animals Humans Vector (molecular biology) lcsh:Science Mice Inbred BALB C Multidisciplinary biology Immunogenicity lcsh:R Vaccination Virology Antibodies Neutralizing 030104 developmental biology chemistry Influenza Vaccines Models Animal biology.protein lcsh:Q Vaccinia |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-14 (2018) |
| ISSN: | 2045-2322 |
| Popis: | Influenza subtypes such as H7 have pandemic potential since they are able to infect humans with severe consequences, as evidenced by the ongoing H7N9 infections in China that began in 2013. The diversity of H7 viruses calls for a broadly cross-protective vaccine for protection. We describe the construction of recombinant modified vaccinia virus Ankara (MVA) vectors expressing the hemagglutinin (HA) or neuraminidase (NA) from three H7 viruses representing both Eurasian and North American H7 lineages – A/mallard/Netherlands/12/2000 (H7N3), A/Canada/rv444/2004 (H7N3), and A/Shanghai/02/2013 (H7N9). These vectors were evaluated for immunogenicity and protective efficacy against H7N3 virus in a murine model of intranasal challenge. High levels of H7-, N3-, and N9-specific antibodies, including neutralizing antibodies, were induced by the MVA-HA and MVA-NA vectors. Mice vaccinated with MVA vectors expressing any of the H7 antigens were protected, suggesting cross-protection among H7 viruses. In addition, MVA vectors expressing N3 but not N9 elicited protection against H7N3 virus challenge. Similar outcomes were obtained when immune sera from MVA vector-immunized mice were passively transferred to naïve mice prior to challenge with the H7N3 virus. The results support the further development of an MVA vector platform as a candidate vaccine for influenza strains with pandemic potential. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|