Decline in protease activities with age in the nematode caenorhabditis elegans
| Autor: | Gary J. Sarkis, John M. Hawdon, Lewis A. Jacobson, James Ashcom |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Aging
medicine.medical_specialty medicine.medical_treatment Proteolysis Immunoblotting Cathepsin D Centrifugation Isopycnic Biology Internal medicine Lysosome medicine Animals Caenorhabditis elegans Cathepsin chemistry.chemical_classification Protease medicine.diagnostic_test Protein turnover biology.organism_classification Molecular biology Endocrinology Enzyme medicine.anatomical_structure chemistry Caenorhabditis Isoelectric Focusing Lysosomes Peptide Hydrolases Developmental Biology |
| Zdroj: | Mechanisms of Ageing and Development. 45:191-201 |
| ISSN: | 0047-6374 |
| DOI: | 10.1016/0047-6374(88)90001-2 |
| Popis: | The activities of 3 lysosomal proteases in the nematode Caenorhabditis elegans are markedly lower in older animals. The aspartyl protease cathepsin D declines about 10-fold from day 3 (early adulthood) to day 11 (near the mean lifespan); this reflects a net decline in the amount of cathepsin D antigen. The specific activity of the thiol protease cathepsin Ce1 declines about 2.5-fold over the same period, and the specific activity of the thiol protease cathepsin Ce2 declines about 8-fold. The activity of a new non-lysosomal protease, designated cathepsin CeX, is invariant with age. The data are consistent with the hypothesis that reduced protease activity in older animals may cause a decline in the rate of protein turnover with age, but do not prove this hypothesis. |
| Databáze: | OpenAIRE |
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