Effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on Akt protein expression is more effective in head and neck cancer cell lineages that retain PTEN protein expression
| Autor: | Andrea Maia Correa Joaquim, Adriana Souza de Jesus, Décio dos Santos Pinto Júnior, Felipe Paiva Fonseca, Hélder Antônio Rebelo Pontes, Ligia Akiko Ninokata Miyahara, Flávia Sirotheau Corrêa Pontes, Lucas Lacerda de Souza |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Lactams Macrocyclic Cell Biology Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Western blot Epidermal growth factor Cell Line Tumor medicine Benzoquinones PTEN Humans Cell Lineage HSP90 Heat-Shock Proteins Protein kinase B medicine.diagnostic_test Epidermal Growth Factor Squamous Cell Carcinoma of Head and Neck PTEN Phosphohydrolase Proto-Oncogene Proteins c-mdm2 medicine.disease Hsp90 Head and neck squamous-cell carcinoma 030104 developmental biology medicine.anatomical_structure Otorhinolaryngology Cell culture Head and Neck Neoplasms 030220 oncology & carcinogenesis biology.protein Cancer research Carcinoma Squamous Cell Periodontics Oral Surgery Tumor Suppressor Protein p53 Proto-Oncogene Proteins c-akt |
| Zdroj: | Journal of oral pathologymedicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology. 47(3) |
| ISSN: | 1600-0714 |
| Popis: | Objectives The aim of this study was to evaluate the expression of Akt, PTEN, Mdm2 and p53 proteins in three different head and neck squamous cell carcinoma (HNSCC) cell lines (HN6, HN19 and HN30), all of them treated with epidermal growth factor (EGF) and 17-allylamino-17-demethoxygeldanamycin (17-AAG), an inhibitor of Hsp90 protein. Material and methods Immunofluorescence and western blot were performed in order to analyze the location and quantification, respectively, of proteins under the action 17-AAG and EGF. Results Treatment with EGF resulted in increased levels of Akt, PTEN and p53 in all cell lineages. The expression of Mdm2 was constant in HN30 and HN6 lineages, while in HN19 showed slightly decreased expression. Under the action 17-AAG, in HN6 and HN19, the expression of PTEN and p53 proteins was suppressed, while Akt and Mdm2 expression was reduced. Finally, in the HN30 cell lineage were absolute absence of expression of Akt, Mdm2 and p53 and decreased expression of PTEN. Conclusion These data allow us to speculate on the particular utility of 17-AAG for HNSCC treatment through the inhibition of Akt protein expression, especially in the cases that retain the expression of PTEN protein. |
| Databáze: | OpenAIRE |
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