Effect of L-Ergothioneine on the metabolic plasma profile of the RUPP rat model of pre-eclampsia
| Autor: | Douglas B. Kell, Fergus P. McCarthy, Rachel D. Williamson, Aude-Claire Morillon, Louise C. Kenny, Philip N. Baker, Jane A. English, Cathal McCarthy |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Antioxidant Maternal Health medicine.medical_treatment Metabolite 030204 cardiovascular system & hematology medicine.disease_cause Biochemistry Rats Sprague-Dawley chemistry.chemical_compound 0302 clinical medicine Pre-Eclampsia Pregnancy Metabolites Medicine and Health Sciences Energy-Producing Organelles Multidisciplinary Organic Compounds Chemistry Obstetrics and Gynecology Lipids Mitochondria 3. Good health Perfusion Physical Sciences Purine Metabolism Metabolome Medicine Steroids Female Metabolic Pathways Cellular Structures and Organelles medicine.symptom Research Article medicine.medical_specialty Science Inflammation Bioenergetics Preeclampsia 03 medical and health sciences In vivo Internal medicine Pressure medicine Animals Sphingolipids Fetus Organic Chemistry Chemical Compounds Biology and Life Sciences Ergothioneine Cell Biology medicine.disease Rats Amino Acid Metabolism Pregnancy Complications Disease Models Animal Metabolism 030104 developmental biology Endocrinology Women's Health Oxidative stress |
| Zdroj: | PLoS ONE, Vol 15, Iss 3, p e0230977 (2020) Morillon, A C, Williamson, R D, Baker, P N, Kell, D B, Kenny, L C, English, J A, McCarthy, F P & McCarthy, C 2020, ' Effect of L-Ergothioneine on the metabolic plasma profile of the RUPP rat model of preeclampsia ', PLOS ONE, vol. 15, no. 3, e0230977 . https://doi.org/10.1371/journal.pone.0230977 PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | IntroductionPre-eclampsia is a major cause of maternal and fetal mortality and morbidity worldwide. Its pathophysiology remains unclear, but mitochondrial dysfunction and oxidative stress have been implicated. L-Ergothioneine is a naturally occurring, water-soluble betaine, that has demonstrated antioxidant properties. Using the reduced uterine perfusion pressure (RUPP) rat model of pre-eclampsia, this study aimed to define the plasma metabolic profile following treatment with L-Ergothioneine.MethodsThe effect of L-Ergothioneine (ET) treatment was explored using in vivo treatment in rats: Sham control (SC, n = 5), RUPP control (RC, n = 5), Sham +ET (ST, n = 5), RUPP +ET (RT, n = 5). Differential expression of plasma metabolites were obtained using untargeted liquid chromatography coupled to mass spectrometry. Statistical analysis was performed on normalised data comparing RC to SC, RT to RC, and RT to ST. Metabolites significantly altered (FDR < 0.05) were identified through database search.ResultsWe report significantly lower levels of L-palmitoylcarnitine in RC compared to SC, a fatty acyl substrate involved in beta-oxidation in the mitochondria. We report that a metabolite that has been associated with oxidative stress (Glutamylcysteine) was detected at significantly higher levels in RT vs RC and RT vs ST. Five metabolites associated with inflammation were significantly lower in RT vs RC and three metabolites in RT vs ST, demonstrating the anti-inflammatory effects of ET in the RUPP rat model of pre-eclampsia.ConclusionsL-Ergothioneine may help preserve mitochondrial function by increasing antioxidant levels, and reducing inflammatory responses associated with pre-eclampsia. This study shows the potential of L-Ergothioneine as a treatment for pre-eclampsia. |
| Databáze: | OpenAIRE |
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