Regulatory elements involved in the tissue-specific expression of the yellow gene of Drosophila
| Autor: | Yuan B. Meng, William N. Chia, Mark Martin |
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| Rok vydání: | 1989 |
| Předmět: |
Transcription
Genetic Restriction Mapping Regulatory Sequences Nucleic Acid Biology Transformation Genetic Drosophilidae Gene expression Genetics Animals Drosophila Proteins Cloning Molecular Promoter Regions Genetic Molecular Biology Gene Regulation of gene expression Base Sequence fungi Intron Nucleic Acid Hybridization Gene rearrangement beta-Galactosidase biology.organism_classification Phenotype Introns Gene Expression Regulation Lac Operon Regulatory sequence Insect Hormones DNA Transposable Elements Drosophila |
| Zdroj: | Molecular and General Genetics MGG. 218:118-126 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/bf00330574 |
| Popis: | We have assessed the DNA sequence requirements for the correct spatial pattern and phenotypic expression of y in the late embryo/larvae. The wild-type larval phenotype requires both the regions between -294 bp and -92 bp and a portion of the intron; the sequence element(s) located within the intron can act in a position independent manner to effect the wild-type larval phenotype. The larval expression pattern was examined by tissue experiments in situ and by staining germline transformants derived from various y/lacZ fusion constructs. The larval expression of y is restricted to the mouthparts, microsetae and anal plates. While the -495 bp to +194 bp region alone cannot effect a wild-type larval expression pattern, this region in conjunction with the intron appears to be sufficient to drive beta-gal expression in an essentially wild-type pattern. Our data further suggest that the -294 bp to -92 bp region contains elements which specify the larval pattern and that the element(s) in the intron normally act to enhance the level of expression necessary for the wild-type larval phenotype. We also present a phenotypic analysis of the adult cuticle structures of germline transformants derived from a variety of deletion and rearrangement constructs of the y gene. This analysis has revealed several new features associated with the regulation of y expression. |
| Databáze: | OpenAIRE |
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